Molecular cloning and expression of AmCDPK from mangroveAvicennia marinaunder elevated temperature
Molecular cloning and expression of AmCDPK from mangroveAvicennia marinaunder elevated temperature
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红树林白骨壤AmCDPK的分子克隆及高温表达
DOI：
10.1007/s10646-020-02204-5
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发表时间：
2020
期刊：
影响因子：
2.7
通讯作者：
Cheng Hao
中科院分区：
文献类型：
--
作者：
Liu Jin;Wang You-Shao;Cheng Hao
钙依赖蛋白激酶（CDPK）家族作为一种重要的钙离子感受器，在陆生植物对生物和非生物胁迫的响应中起着重要的作用。研究表明白骨壤具有较好的耐热性。从红树林A.经RACE-PCR扩增，命名为AmCDPK。通过预测和分析其性质、结构和表达模式，发现其氨基酸序列与Handroanthusimpetiginosus和Sesamumindicum高度同源，含有一个激酶结构域和4个EF-手形Ca ~（2+）结合位点。实时荧光定量PCR分析结果表明，AmCDPK在热应激条件下表达显著上调。通过对AmCDPK基因顺式元件的分析，推测AmCDPK基因可能是一个多功能基因，参与了脱水、低温、光照、防御和阿坝胁迫反应。这是首次从红树植物中克隆出CDPKs，为AmCDPKs在热胁迫中的作用提供了证据，在全球变暖的背景下，AmCDPKs在热胁迫中的作用显得尤为重要。
Considered as an essential calcium sensor, the calcium-dependent protein kinase (CDPK) family plays a critical part in terrestrial plants’ responses to both biotic and abiotic stresses. In the study,Avicennia marinawas proved to have better heat tolerance than other species. ACDPKgene was cloned from mangrove speciesA. marinausing RACE-PCR and designated asAmCDPK. By predicting and analyzing its properties, structures and expression patterns, we found that the amino acid sequence, containing a kinase domain and four EF-hand Ca2+-binding sites, shared high identity withHandroanthus impetiginosusandSesamum indicum. Quantitative real-time PCR data analysis suggested thatAmCDPKdemonstrated significant up-regulation under heat stress. It is likely thatAmCDPKis a versatile gene involved in various stresses, including dehydration, cold, light, defense and ABA stress responses by analyzingcis-elements. It is the first time thatCDPKs from mangroves have been cloned and our results brought evidence to the effect ofAmCDPKon heat stress, which is particularly important under the background of global warming.