Analysis of single nucleic acid molecules with protein nanopores.
Analysis of single nucleic acid molecules with protein nanopores.
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DOI：
10.1016/s0076-6879(10)75022-9
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发表时间：
2010
影响因子：
--
通讯作者：
Bayley, Hagan
中科院分区：
文献类型：
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作者：
Maglia, Giovanni;Heron, Andrew J.;Stoddart, David;Japrung, Deanpen;Bayley, Hagan
我们描述了在我们的实验室中用于分析具有蛋白质纳米孔的单个核酸分子的方法。技术部分之前是各种实验，可以做蛋白质纳米孔的回顾。这项工作的最终目标是单分子DNA测序，尽管测序在这里没有明确讨论。技术部分包括核酸分析所需的设备、必要材料的制备和储存、信号处理和数据分析等方面。
We describe the methods used in our laboratory for the analysis of single nucleic acid molecules with protein nanopores. The technical section is preceded by a review of the variety of experiments that can be done with protein nanopores. The end goal of much of this work is single-molecule DNA sequencing, although sequencing is not discussed explicitly here. The technical section covers the equipment required for nucleic acid analysis, the preparation and storage of the necessary materials, and aspects of signal processing and data analysis.