Neurotrophic and neuroprotective actions of Achyranthes bidentata polypeptides on cultured dorsal root ganglia of rats and on crushed common peroneal nerve of rabbits
Neurotrophic and neuroprotective actions of Achyranthes bidentata polypeptides on cultured dorsal root ganglia of rats and on crushed common peroneal nerve of rabbits
复制标题
牛膝多肽对培养的大鼠背根神经节和损伤的兔腓总神经的神经营养和神经保护作用
DOI：
10.1016/j.neulet.2013.12.015
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发表时间：
2014-03-06
影响因子：
2.5
通讯作者：
Ding, Fei
中科院分区：
文献类型：
--
作者：
Cheng, Qiong;Yuan, Ying;Ding, Fei
从怀牛膝水提液中分离得到牛膝多肽（ABPP）。bidenrata Blume，一种具有多种治疗用途的传统中药。本研究旨在观察ABPP对体外培养的大鼠背根神经节（DRG）的神经营养作用和对兔腓总神经损伤的神经保护作用。免疫化学和蛋白质印迹分析表明，ABPP（0.01，0.1，和1.0 μ g/ml）通过激活ERK 1/2，以浓度依赖性的方式鼓励从培养的DRG外植体/神经元的轴突生长。兔腓总神经挤压伤后，每天给予ABPP，持续5周。电生理和组织形态学评价表明，6.0 mg/kg ABPP显著促进神经再生和功能恢复。我们的研究结果表明，ABPP可以作为一种神经营养和神经保护剂，治疗周围神经挤压伤。(C)2013爱思唯尔爱尔兰有限公司版权所有。
We have isolated Achyranthes bidentata Blume polypeptides (ABPP) from the aqueous extract of A. bidenrata Blume, a traditional Chinese medicine with multiple therapeutic applications. In this study, we aimed to investigate neurotrophic effects of ABPP on cultured dorsal root ganglia (DRGs) of rats and neuroprotective effects on crushed common peroneal nerve of rabbits. Immunochemistry and Western blot analysis indicated that ABPP (0.01, 0.1, and 1.0 mu g/ml) encouraged neurite outgrowth from cultured DRG explants/neurons in a concentration-dependent manner through activation of ERK1/2. After crush injury to rabbit common peroneal nerve, animals received daily administration of ABPP for 5 weeks. Electro-physiological assessments and histomorphological evaluation showed that 6.0 mg/kg ABPP significantly enhanced nerve regeneration and function restoration. Our findings suggest that ABPP could be used as a neurotrophic and neuroprotective agent to treat peripheral nerve crush injury. (C) 2013 Elsevier Ireland Ltd. All rights reserved.