Effect of castration and sex hormone treatment on survival, anti-nucleic acid antibodies, and glomerulonephritis in NZB/NZW F1 mice.
Effect of castration and sex hormone treatment on survival, anti-nucleic acid antibodies, and glomerulonephritis in NZB/NZW F1 mice.
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DOI：
10.1084/jem.147.6.1568
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发表时间：
1978-06-01
期刊：
影响因子：
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通讯作者：
Siiteri PK
中科院分区：
文献类型：
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作者：
Roubinian JR;Talal N;Greenspan JS;Goodman JR;Siiteri PK
NZB/NZW F1 两种性别的小鼠在 2 周龄时被阉割，并皮下植入含有 5-α-二氢睾酮或雌二醇-17-β 的硅橡胶管。通过光、免疫荧光和电子显微镜测定，接受雄激素的小鼠表现出存活率提高、抗核酸抗体减少或肾小球肾炎证据减少。相比之下，在接受雌激素的阉割小鼠中观察到相反的效果。完整的雄性 NZB/NZW F1 小鼠在 8 个月时接受雄激素植入，在这个年龄，它们会加速发展与血清睾酮浓度下降相关的自身免疫性疾病。经过此类治疗的小鼠的存活率得到提高，DNA 和聚腺苷酸 (Poly A) 抗体浓度降低。雄性 NZB/NZW F1 小鼠的青春期前去势会导致 Poly A 的 IgG 抗体更早出现。如果还进行了新生儿胸腺切除术，则可以防止这种去势效应。
NZB/NZW F1 mice of both sexes were castrated at 2 wk of age and implanted subcutaneously with silastic tubes containing either 5-alpha- dihydrotestosterone or estradiol-17-beta. Mice receiving androgen showed improved survival, reduced anti-nucleic acid antibodies, or less evidence of glomerulonephritis as determined by light, immunofluorescent, and electron microscopy. By contrast, opposite effects were observed in castrated mice receiving estrogen. Intact male NZB/NZW F1 mice received androgen implants at 8 mo, an age when they develop an accelerated autoimmune disease associated with a decline in serum testosterone concentration. Such treated mice had improved survival and reduced concentrations of antibodies to DNA and to polyadenylic acid (Poly A). Prepubertal castration of male NZB/NZW F1 mice results in an earlier appearance of IgG antibodies to Poly A. This effect of castration was prevented if neonatal thymectomy was also performed.