Liver denervation affects hepatocyte mitochondrial fatty acid transport capacity
Liver denervation affects hepatocyte mitochondrial fatty acid transport capacity
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DOI：
10.1002/cbf.1047
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发表时间：
2004-01-01
影响因子：
3.6
通讯作者：
Seelaender, MCL
中科院分区：
文献类型：
--
作者：
Carreño, FR;Seelaender, MCL
本文研究了大鼠肝脏去神经支配对肝脏肉毒碱棕榈酰转移酶（CPT）系统活性的影响，该系统催化长链脂肪酸向线粒体的转移。去酚神经支配的肝脏（D）中去甲肾上腺素含量降低了87%。CPT I和II活动（通过去污剂分离酶后的放射性测定法测量）降低D组（p < 0.001）（分别为2.6 +/- 0.1和0.68 +/- 0.2 nmol min（-1）mg（-1）蛋白质），与对照组相比（CPT I和II分别为4.7 +/- 0.3和2.5 +/- 0.2 nmol min（-1）mg（-1）蛋白）。免疫印迹法后获得的一个较弱的免疫反应带去神经肝CPT IIas。同时，与对照组相比，D组[（14）C]-油酸酯给药后评价的长链脂肪酸掺入（p <0.001）和总脂肪含量（p < 0.001）增加，而外源性[（14）C]-油酸酯掺入分泌的VLDL减少（p < 0.01）。与完整大鼠肝脏相比，去交感神经对CPT活性的影响与去肾上腺髓质引起的CPT活性增强不同（p < 0.01）。去神经支配和肾上腺髓质化对其他脂质代谢参数的影响是相似的。结果强烈表明肝脏交感神经支配在调节肝脏脂质代谢中的作用。版权所有（C）2003约翰威利父子有限公司。
The effect of liver denervation on the activity of hepatic carnitine palmitoyltransferase (CPT) system, which catalyses the transfer of long-chain fatty acids into the mitochondria, was studied in rats. Noradrenaline content in phenol-denervated liver (D) was reduced by 87%. CPT I and II activities (measured by radioassay after detergent separation of the enzymes) were decreased (p < 0.001) in D (2.6 +/- 0.1 and 0.68 +/- 0.2 nmol min(-1) mg(-1) protein, respectively) as compared with controls (4.7 +/- 0.3 and 2.5 +/- 0.2 nmol min(-1) mg(-1) protein, for CPT I and II, respectively). A less intense immunoreactive band for denervated liver CPT IIas obtained after Western blotting. Concomitantly, long-chain fatty acid incorporation (p 0.001), evaluated after administration of [(14)C]-oleate and total fat content (p < 0.001) were increased in D in relation to controls, while incorporation of exogenous [(14)C]-oleate into secreted VLDL, was decreased (p < 0.01). The effect of sympathetic denervation on CPT activity was different from that evoked by adrenodemedullation, which caused an augmentation of CPT activity (p < 0.01), when compared with the liver of intact rats. The effects of denervation and adrenodemedullation on the other parameters of lipid metabolism studied, were similar. The results strongly suggest a role of liver sympathetic innervation in the regulation of liver lipid metabolism. Copyright (C) 2003 John Wiley Sons, Ltd.