PERIODIC CROSS-LINKING OF MICROTUBULES BY CYTOPLASMIC MICROTUBULE-ASSOCIATED AND MICROTUBULE-CORSET PROTEINS FROM A TRYPANOSOMATID
PERIODIC CROSS-LINKING OF MICROTUBULES BY CYTOPLASMIC MICROTUBULE-ASSOCIATED AND MICROTUBULE-CORSET PROTEINS FROM A TRYPANOSOMATID
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DOI：
10.1073/pnas.84.10.3259
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发表时间：
1987-05-01
影响因子：
11.1
通讯作者：
FLAVIN, M
中科院分区：
文献类型：
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作者：
BRAMBLETT, GT;CHANG, S;FLAVIN, M
束状crithidiafasciculata的细胞骨架中的主要元素是包围细胞体并紧密位于质膜下面的微管的外周紧身衣。通过交联保持50 nm的横向间距，并且微管可以连接到质膜。我们已经表征了与从细胞质中体外聚合的微管相关的多肽组，或者与紧身胸衣复合物相关的多肽组。除了两组中存在的一个Mr 33，000外，它们不同，相应的天然紧身衣蛋白似乎是Mr 66，000的二聚体。这些蛋白质与脑微管蛋白共聚，所得聚合物由微管对或小平行束组成，通过间隔约8.5 nm的周期性交联连接。
The dominant element in the cytoskeleton of Crithidia fasciculata is a peripheral corset of microtubules enclosing the cell body and closely underlying the plasma membrane. A lateral spacing of 50 nm is maintained by crosslinks, and microtubules may be linked to the plasma membrane. We have characterized groups of polypeptides that associate with microtubules polymerized in vitro from the cytoplasm, or that are associated with the corset complex. They differ except for one of Mr 33,000 present in both groups, The corresponding native corset protein appears to be a dimer of Mr 66,000. These protein(s) copolymerize with brain tubulin, and the resultant polymer consists of pairs or small parallel bundles of microtubules, joined by periodic crosslinks spaced about 8.5 nm apart.