Structure and function of the Bacillus SpoIIE protein and its localization to sites of sporulation septum assembly
Structure and function of the Bacillus SpoIIE protein and its localization to sites of sporulation septum assembly
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DOI：
10.1046/j.1365-2958.1996.433963.x
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发表时间：
1996-03-01
影响因子：
3.6
通讯作者：
Youngman, P
中科院分区：
文献类型：
--
作者：
Barak, I;Behari, J;Youngman, P
枯草芽孢杆菌spollE基因座的功能是在孢子形成过程中形成正常的极隔膜和激活转录因子所必需的，该转录因子指导早期前孢子特异性基因的表达。我们已经确定了B的spollE基因的野生型和几个突变等位基因的DNA序列。枯草芽孢杆菌和测序的相当大的一部分，其假定的同源物在巨大芽孢杆菌，我们表明，spollE基因座编码一个单一的大蛋白质与预测的分子量为92 kDa。传统上定义基因座的五个点突变等位基因和两个转座子产生的突变中的每一个都显示落在92 kDa基因产物的编码序列内或落在预期其表达所需的序列内。预测的SpollE基因产物的氨基末端部分，包含约40%的蛋白质，具有极强的疏水性，预计含有多达12个跨膜片段，蛋白质的其余部分不含足够长以跨越脂双层的疏水片段，因此推测包含一个或多个球状水相暴露结构域，连接B的3'端的框内融合。将巨大芽孢杆菌spollE编码序列插入到gfp（维多利亚水生藻的绿色荧光蛋白（GFP）的编码基因）的5'末端，导致了定位于孢子形成隔膜组装位点的强烈的孢子形成特异性荧光信号。我们推测SpollE在孢子形成隔膜组装中起作用，可能决定了允许发育期间隔室间信号传导的结构的特殊性质。
Functioning of the spollE locus of Bacillus subtilis is required for formation of a normal polar septum during sporulation and for activation of the transcription factor of, which directs early forespore-specific gene expression, We have determined the DNA sequence of the wild type and several mutant alleles of the spollE gene of B. subtilis and sequenced a substantial portion of its presumptive homologue in Bacillus megaterium, We show that the spollE locus encodes a single large protein with a predicted molecular mass of 92 kDa. Each of five point-mutation alleles, which have traditionally defined the locus, and two transposon-generated mutations were shown to fall within the coding sequence for the 92 kDa gene product or within sequences expected to be required for its expression, The aminoterminal portion of the predicted SpollE gene product, comprising approximately 40% of the protein, is extremely hydrophobic and is expected to contain up to 12 membrane-spanning segments, The remainder of the protein contains no hydrophobic segments long enough to span a lipid bilayer and is therefore presumed to comprise one or more globular, aqueous-phase exposed domains, An in-frame fusion joining the 3' end of the B. megaterium spollE coding sequence to the 5' end of gfp, a gene encoding the green fluorescent protein (GFP) of Aquorea victoria, resulted in a strong, sporulation-specific fluorescent signal localized to the sites of sporulation septum assembly, We speculate that SpollE plays a role in assembling the sporulation septum, perhaps determining the special properties of the structure that permit intercompartment signalling during development.