GENESIS OF RODS IN TELEOST FISH RETINA
GENESIS OF RODS IN TELEOST FISH RETINA
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DOI：
10.1038/293141a0
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发表时间：
1981-01-01
期刊：
影响因子：
64.8
通讯作者：
FERNALD, RD
中科院分区：
文献类型：
--
作者：
JOHNS, PR;FERNALD, RD
鱼一生都在生长，随着眼睛尺寸的增大，新的神经元被添加到视网膜上。随着视网膜的扩张，细胞密度降低：神经节细胞、视锥细胞和内核层细胞在较大的（老的）鱼的视网膜中间隔得更远2- 5,7 -9。相反，视杆细胞的密度在幼虫发育过程中增加，然后随着成虫眼睛的生长而大致保持不变。在之前的发育研究中，3h -胸腺嘧啶被用来鉴定增殖细胞，发现在视网膜边缘有一个生发区。生发细胞分裂产生新的视网膜神经元，这些神经元环状地附着在正在生长的视网膜周围5,6,10。在两栖类幼虫中也发现了类似的环状生长模式11-15。这些研究表明视网膜边缘是胚胎后视网膜神经发生的唯一部位。相反，我们的观察表明，新的视杆细胞起源于前体细胞的有丝分裂，前体细胞散布在视网膜内成熟视杆细胞的细胞核中。视网膜上的视杆细胞的选择性增加可以解释当鱼的视网膜生长时，视杆细胞相对于其他神经元的比例是如何增加的。这些实验的初步报告已经出现在其他地方。
Abstract Fish grow throughout life1and new neurones are added to the retina as the eyes increase in size2-6. As the retina expands, the density of cells decreases: ganglion cells, cones and cells in the inner nuclear layer are spaced further apart in retinas from larger (older) fish2-5, 7-9. In contrast, the density of rods increases during larval development and is then maintained approximately constant as the adult eye grows2-5. Previous developmental studies, in which3H-thymidine was used to identify proliferating cells, revealed a germinal zone at the margin of the retina. The germinal cells divide to produce new retinal neurones which are added annularly at the perimeter of the growing retina5, 6, 10. A similar circumferential pattern of growth has been demonstrated in larval amphibians11-15. These studies concluded that the retinal margin is the only site of neurogenesis in post-embryonic retinas. In contrast, our observations suggest that new rods originate from mitotic divisions of precursor cells which are interspersed among the nuclei of mature rods within the retina. The selective addition of rods throughout the retina could explain how the proportion of rods relative to other neurones increases as the retinas of fish grow2-4, 7. Preliminary reports of these experiments have appeared elsewhere16, 17.