In situ detecting changes in membrane lipid phenotypes of macrophages cultured in different cancer microenvironments using mass spectrometry
In situ detecting changes in membrane lipid phenotypes of macrophages cultured in different cancer microenvironments using mass spectrometry
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使用质谱原位检测不同癌症微环境中培养的巨噬细胞膜脂表型的变化
DOI：
10.1016/j.aca.2018.04.036
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发表时间：
2018
影响因子：
6.2
通讯作者：
Zhili Li
中科院分区：
文献类型：
--
作者：
Yupin Xu;Mo Zhang;Qing Wang;Zhili Li
巨噬细胞是免疫系统的重要细胞，在不同的微环境中表现出独特的基因表型和不同的功能。在本研究中，巨噬细胞RAW264.7（M0巨噬细胞）和脂多糖（LPS）加干扰素γ（INF-γ）处理的M0巨噬细胞（M1巨噬细胞）在不同的肺细胞来源的培养上清液（CS）中培养作为模拟肿瘤微环境。通过基质辅助激光解吸/电离-傅里叶变换离子回旋共振质谱法原位检测完整巨噬细胞的脂质（主要来自细胞膜）。在负离子模式下观察到大约 300 个小分子。偏最小二乘判别分析（PLS-DA）表明两种类型的巨噬细胞具有不同的膜脂表型。磷脂酰乙醇胺PE(16:1/18:0)、PE(18:1/18:0)、PE(36:2)、PE-Cer(d36:1)和PE(P-16:0/18:1)水平的变化与巨噬细胞膜表型密切相关。热图还显示，与不同的肺细胞来源的 CS 相比，体外定向诱导经典激活的巨噬细胞（M1 巨噬细胞）对巨噬细胞的膜脂表型具有更大的影响。结果与生物技术获得的数据一致。
Macrophages, the important cells of immune system, have exhibited distinct gene phenotypes with diverse functions in different microenvironments. In the present study, macrophages RAW264.7 (M0 macrophages) and lipopolysaccharide (LPS) plus interferon gamma (INF-γ)-treated M0 macrophages (M1 macrophages) were cultured in different lung cell-derived culture supernatants (CSs) as imitative tumor microenvironments. The lipids (mainly from cell membrane) of intact macrophages werein situdetected by matrix-assisted laser desorption/ionization-Fourier transform ion cyclotron resonance mass spectrometry. Approximately 300 of small molecules were observed in negative ion mode. Partial least square-discriminant analysis (PLS-DA) suggested that two types of the macrophages have different membrane lipid phenotypes. Changes in the levels of phosphatidylethanolamine PE(16:1/18:0), PE(18:1/18:0), PE(36:2), PE-Cer(d36:1), and PE(P-16:0/18:1) were closely associated with membrane phenotypes of macrophages. The heatmap also revealed that directional induction to classically activated macrophages (M1 macrophages) in vitro had greater impact on the membrane lipid phenotypes of macrophages than different lung cell-derived CSs. The results are consistent with the data obtained by biological technologies.