Identification and characterization of the leaf specific networks of inner and rosette leaves in Brassica rapa
Identification and characterization of the leaf specific networks of inner and rosette leaves in Brassica rapa
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DOI：
10.1016/j.bbrc.2017.06.123
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发表时间：
2017-08-26
影响因子：
3.1
通讯作者：
Lim, Yong Pyo
中科院分区：
文献类型：
--
作者：
Kim, Man -Sun;Hong, Seongmin;Lim, Yong Pyo
白菜的内叶和莲座叶在营养价值、外观、口感、色泽和质地等方面具有不同的特点。许多研究者利用差异表达基因来研究芜菁内叶和莲座叶的差异。然而，基因的功能特征是由基因之间复杂的相互作用决定的。因此，需要一个高贵的网络方法来阐明这种功能差异，而不是单独通过基因表达谱捕获。在这项研究中，我们测量了基因表达的标准甘蓝基因组中的RNA测序和构建莲座和内叶网络的基因表达谱的基础上。此外，我们比较了这些网络的拓扑和功能特性。我们发现莲座丛和内叶网络之间的功能差异显着。具体而言，我们发现莲座叶网络中的基因与稳态和对外界刺激的反应有关，而内部叶网络中的基因主要与谷氨酰胺生物合成过程和激素的发育过程有关。总的来说，网络方法提供了一个洞察两个叶子的功能差异。(C)2017爱思唯尔公司All rights reserved.
Inner and rosette leaves of Chinese cabbage (Brassica rapa) have different characteristics in terms of nutritional value, appearance, taste, color and texture. Many researchers have utilized differentially expressed genes for exploring the difference between inner and rosette leaves of Brassica rapa. The functional characteristics of a gene, however, is determined by complex interactions between genes. Hence, a noble network approach is required for elucidating such functional difference that is not captured by gene expression profiles alone. In this study, we measured gene expression in the standard cabbage genome by RNA-Sequencing and constructed rosette and inner leaf networks based on the gene expression profiles. Furthermore, we compared the topological and functional characteristics of these networks. We found significant functional difference between the rosette and inner leaf networks. Specifically, we found that the genes in the rosette leaf network were associated with homeostasis and response to external stimuli whereas the genes in the inner leaf network were mainly related to the glutamine biosynthesis processes and developmental processes with hormones. Overall, the network approach provides an insight into the functional difference of the two leaves. (C) 2017 Elsevier Inc. All rights reserved.