Vasoactive intestinal polypeptide stimulates cell proliferation and adenylate cyclase activity of cultured human keratinocytes.
Vasoactive intestinal polypeptide stimulates cell proliferation and adenylate cyclase activity of cultured human keratinocytes.
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血管活性肠多肽刺激培养的人角质形成细胞的细胞增殖和腺苷酸环化酶活性。
DOI：
10.1073/pnas.86.15.5993
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发表时间：
1989
影响因子：
11.1
通讯作者：
J. Nilsson
中科院分区：
文献类型：
--
作者：
A. Haegerstrand;B. Jonzon;C. Dalsgaard;J. Nilsson
越来越多的证据表明周围神经的营养作用。本研究观察了感觉神经肽血管活性肠肽（VIP）、P物质（SP）、降钙素基因相关肽（CGRP）和生长抑素（SOM）对体外培养的人角质形成细胞的生长刺激作用。研究表明，在致死处理的3 T3成纤维细胞饲养细胞和表皮生长因子（EGF）存在的情况下，VIP以剂量依赖性方式刺激角质形成细胞的增殖，而SP、CGRP和SOM则无效。VIP刺激腺苷酸环化酶活性的膜从培养的角质形成细胞以剂量依赖性的方式，表明参与cAMP作为第二信使在这个反应。此外，125I标记的VIP被证明与培养的角质形成细胞结合，这种结合可以通过添加未标记的VIP来取代，这表明存在特异性受体。因此，从皮肤中的感觉神经末梢释放的VIP可能通过经由特异性VIP受体刺激腺苷酸环化酶活性而充当人角质形成细胞的局部促有丝分裂因子。
An increasing body of evidence has suggested trophic effects of peripheral nerves. In this study, the growth stimulatory properties of the sensory neuropeptides vasoactive intestinal polypeptide (VIP), substance P (SP), calcitonin generelated peptide (CGRP), and somatostatin (SOM) on cultured human keratinocytes were investigated. It was shown that VIP, in the presence of lethally treated 3T3 fibroblast feeder cells and epidermal growth factor (EGF), stimulated proliferation of keratinocytes in a dose-dependent manner, whereas SP, CGRP, and SOM were ineffective. VIP stimulated adenylate cyclase activity in membranes obtained from cultured keratinocytes in a dose-dependent manner, indicating an involvement of cAMP as second messenger in this reaction. Furthermore, 125I-labeled VIP was shown to bind to cultured keratinocytes and this binding could be displaced by addition of unlabeled VIP, suggesting the presence of specific receptors. It is therefore possible that VIP, released from sensory nerve endings in the skin, may act as a local mitogenic factor for human keratinocytes by stimulating adenylate cyclase activity via specific VIP receptors.