Analysis of tobacco-specific N-nitrosamines in snuff by ethyl acetate extraction and liquid chromatography-tandem mass spectrometry.
Analysis of tobacco-specific N-nitrosamines in snuff by ethyl acetate extraction and liquid chromatography-tandem mass spectrometry.
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DOI：
10.1016/s0021-9673(03)00981-6
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发表时间：
2003-08
期刊：
影响因子：
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通讯作者：
C. Jansson;A. Paccou;B. Osterdahl
中科院分区：
文献类型：
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作者：
C. Jansson;A. Paccou;B. Osterdahl
建立了鼻烟中4种烟草特有的N-亚硝胺（N′-亚硝基去甲烟碱、N ′-亚硝基新烟草碱、N ′-亚硝基假木贼碱和4-（甲基亚硝胺基）-1-（3-吡啶基）-1-丁酮）的快速、高选择性、高灵敏度的分析方法。采用乙酸乙酯萃取分离亚硝胺，并采用LC-MS-MS进行分析。除蒸发和过滤外，在所提出的方法中不需要额外的净化步骤。溶剂中标准品的检测限为0.0005 - 0.001 μg/ml（0.005 - 0.01 μg/g）。
A rapid, selective and sensitive method for routine analysis of the four tobacco-specific N-nitrosamines, N′-nitrosonornicotine, N′-nitrosoanatabine, N′-nitrosoanabasine and 4-(methyl-nitrosamino)-1-(3-pyridyl)-1-butanone in snuff has been developed. The nitrosamines were isolated by ethyl acetate extraction and analysed by LC–MS–MS. Except for evaporation and filtration, no additional clean-up steps are needed in the proposed method. The detection limits for standard in solvent are between 0.0005 and 0.001 μg/ml (0.005 and 0.01 μg/g).