Improvement of citric acid production by Aspergillus niger with addition of phytate to beet molasses

Improvement of citric acid production by Aspergillus niger with addition of phytate to beet molasses
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DOI:
10.1016/s0960-8524(98)00026-1
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发表时间:
1998-09-01
影响因子:
11.4
通讯作者:
Wang, JL
Wang, JL
中科院分区:
工程技术1区
文献类型:
--
作者:
Wang, JL

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植酸盐是一种重要的植物成分,可以在谷物和豆类的种子中找到。植酸在植酸分子中有12个可替换的质子,使其能够与多价阳离子络合。以植酸盐为添加剂,对黑曲霉利用未经处理的啤酒糖蜜发酵生产柠檬酸进行了研究。植酸的添加提高了还原糖的利用率,促进了柠檬酸的积累,但对菌丝生长有一定的抑制作用。植酸盐添加的效果被认为是依赖于浓度和添加植酸盐的发酵阶段。当在孵育开始时添加时,培养基中用于柠檬酸生产的植酸盐的最佳浓度为10 g/l,这导致柠檬酸积累增加约3.1倍。在发酵过程中,向培养基中添加16 g/l植酸盐,孵育3天后得到最大柠檬酸产量,其比对照实验高约2.4倍。(C)1998爱思唯尔科技有限公司版权所有。
Phytate is an important plant constituent and can be found in the seeds of cereals and legumes..Phytic acid has 12 replaceable protons in the phytic molecule, giving it the ability to complex with multivalent cations. In this study, the phytate was used as an additive to enhance citric acid production by Aspergillus niger from an untreated beer molasses. The addition of phytate caused increase of reducing sugar utilization and enhanced citric acid accumulation; however the mycelial growth was slightly inhibited. The effect of phytate addition was found to be dependent on concentration and the stage of fermentation at which phytate was added. When added at the beginning of incubation, the optimal concentration of phytate in the medium for citric acid production was 10 g/l, which resulted in about a 3.1-fold increase in citric acid accumulation. During the fermentation addition of 16 g/l phytate to the medium, after 3 days incubation gave the maximal citric acid production which was about 2.4-fold higher than the control experiment. (C) 1998 Elsevier Science Ltd. All rights reserved.