Metabolomic stability of exercise-induced sweat
Metabolomic stability of exercise-induced sweat
复制标题
DOI：
10.1016/j.jchromb.2019.121763
复制
发表时间：
2019-09-15
影响因子：
3
通讯作者：
Martin, Jennifer A.
中科院分区：
文献类型：
--
作者：
Harshman, Sean W.;Pitsch, Rhonda L.;Martin, Jennifer A.
由于人们对利用汗液进行生物标志物发现的兴趣越来越大，必须生成支持样本收集和处理方法的数据，以便进行受控的大规模生物标志物发现研究。在这篇论文中，从室温或37摄氏度的模拟体温下保持90分钟的运动诱导的排泄汗液中定量测定了12种氨基酸。数据表明，汗液中氨基酸之间存在很大的动态范围。此外，不同个体和同一个体在不同贮藏条件下的氨基酸含量也存在差异，其中丙氨酸、精氨酸和苏氨酸在不同采样事件之间差异显著（p < 0.05）。此外，结果表明，在两种测试的储存温度下，氨基酸相对不变，长达90分钟，< 10%（15/156）的氨基酸测量显示，从时间零值变化大于10%。一种非靶向代谢组学方法也被应用于数据集，以评估代谢组学的整体变化。结果表明，无论温度条件和电离模式如何，超过88%的数据点落在既定范围内。总的来说，这项研究表明，在长达90分钟的两种不同温度下，汗液在很大程度上是不变的。这些结果建立了汗液收集和样品处理可能长达90分钟，代谢物丰度变化极小。
Due to increased interest in the use of excreted sweat for biomarker discovery, data must be generated supporting sample collection and handling methods to allow for controlled, large-scale biomarker discovery studies to be performed. In this manuscript, twelve amino acids were quantitated from exercise-induced excreted sweat held at room temperature or a simulated body temperature of 37 degrees C for up to 90 min. The data illustrate a large dynamic range exists among amino acids in sweat. Additionally, the amino acid quantities vary across individuals and among the same individual under different storage conditions, with alanine, arginine, and threonine showing a significant statistical difference between sampling events (p < 0.05). Furthermore, the results establish amino acids are relatively invariant, at both storage temperatures tested, for up to 90 min illustrated by < 10% (15/156) of the amino acids measurements demonstrating change greater than 10% from the time zero value. An untargeted metabolomics approach was also applied to the data set to evaluate global changes to the metabolome. The results show more than 88% of all data points fall within the established limits, regardless of temperature condition and ionization mode. Collectively, this study demonstrates that sweat is largely invariant at two distinct temperatures for up to 90 min. These results establish sweat collection and sample handling is possible for up to 90 min with minimal changes in metabolite abundances.