A DNA-Based Candidate HIV Vaccine Delivered via In Vivo Electroporation Induces CD4 Responses toward the α4β7-Binding V2 Loop of HIV gp120 in Healthy Volunteers
A DNA-Based Candidate HIV Vaccine Delivered via In Vivo Electroporation Induces CD4 Responses toward the α4β7-Binding V2 Loop of HIV gp120 in Healthy Volunteers
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DOI：
10.1128/cvi.00327-12
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发表时间：
2012-09-01
影响因子：
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通讯作者：
Vasan, Sandhya
中科院分区：
文献类型：
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作者：
Kopycinski, Jakub;Cheeseman, Hannah;Vasan, Sandhya
通过体内电穿孔 (EP) 施用 C/B' 分支候选 HIV-1 DNA 疫苗 ADVAX 是安全的，并且比不进行 EP 的肌肉注射更具免疫原性。 T 细胞对全长 Env 反应的广度和特异性被绘制出来。诱导了对多个 Env 区域的反应，其中大多数集中在 V3/C4 和 V2 区域，包括 α4β7 整联蛋白结合域。这种 DNA 疫苗方案诱导的反应范围与病毒载体疫苗方案相当。
Administration of a clade C/B' candidate HIV-1 DNA vaccine, ADVAX, by in vivo electroporation (EP) was safe and more immunogenic than intramuscular administration without EP. The breadth and specificity of T-cell responses to full-length Env were mapped. Responses to multiple Env regions were induced, with most focusing on V3/C4 and V2 regions, including the alpha 4 beta 7 integrin-binding domain. The breadth of responses induced by this DNA vaccine regimen was comparable to that of viral-vectored vaccine regimens.