Transient increase in salicylic acid and its glucose conjugates after wounding in Arabidopsis leaves
Transient increase in salicylic acid and its glucose conjugates after wounding in Arabidopsis leaves
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拟南芥叶片受伤后水杨酸及其葡萄糖结合物的短暂增加
DOI：
10.5511/plantbiotechnology.27.205
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发表时间：
2010
影响因子：
1.6
通讯作者：
D. Shibata
中科院分区：
文献类型：
--
作者：
Takumi Ogawa;T. Ara;K. Aoki;Hideyuki Suzuki;D. Shibata
我们分析了拟南芥叶片机械损伤后的代谢变化，以揭示损伤对代谢的影响。通过用碳化硅颗粒（carboresin）摩擦叶表面来损伤拟南芥叶，如用于实验病毒感染，并放置长达48小时。我们用超高效液相色谱-飞行时间质谱法分析了五个重复样品在创伤后每个时间点的代谢物。茉莉酸的生产诱导受伤后立即，如前所述。有趣的是，我们发现水杨酸的量在创伤后6小时开始显著增加，随后在创伤后24小时水杨酸葡糖苷和水杨酸葡萄糖酯瞬时增加。水杨酸生物合成途径中基因的表达模式可在公共转录组数据库中获得，也支持水杨酸及其葡萄糖缀合物的代谢变化的观察。这些结果表明创伤后水杨酸代谢的活化，表明水杨酸在创伤愈合中的作用。本研究获得的代谢组数据可从MassBase代谢组数据库（http：//webs2.kazusa.or.jp/massbase/）获得。
We analyzed metabolic changes after mechanical wounding in Arabidopsis leaves to reveal wound effects on metabolism. Arabidopsis thaliana leaves were wounded by rubbing the leaf surface with silicon carbide particles (carborundum), as for experimental viral infection, and left up to 48 hours. We analyzed the metabolites of five replicate samples at each time point after wounding by ultra performance liquid chromatography time-of-flight mass spectrometry. Jasmonic acid production was induced immediately after wounding, as reported previously. Interestingly, we found that the amount of salicylic acid started to increase significantly 6 hours after wounding, followed by transient increases in salicylic acid glucoside and salicylic acid glucose ester 24 hours after wounding. The expression patterns of genes in salicylic acid biosynthesis pathway, which are available at public transcriptome databases, also support the observation of metabolic changes in salicylic acid and its glucose conjugates. These results indicate activation of salicylic acid metabolism after wounding, suggesting a role of salicylic acid in wound healing. The metabolome data obtained from this study is available from the MassBase metabolome database (http://webs2.kazusa.or.jp/massbase/).
影响因子：
2.8
作者：
Ratzinger, Astrid;Riediger, Nadine;von Tiedemann, Andreas;Karlovsky, Petr
通讯作者：
Karlovsky, Petr