The effect of actin disrupting agents on contact guidance of human embryonic stem cells
The effect of actin disrupting agents on contact guidance of human embryonic stem cells
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DOI：
10.1016/j.biomaterials.2007.05.027
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发表时间：
2007-10-01
期刊：
影响因子：
14
通讯作者：
Langer, Robert
中科院分区：
文献类型：
--
作者：
Gerecht, Sharon;Bettinger, Christopher J.;Langer, Robert
哺乳动物细胞通过基因表达谱、形态、增殖和迁移的复杂变化对其底物作出反应。我们报告说，基板纳米形貌改变人类胚胎干细胞（hESC）的形态和增殖。纤连蛋白包被的聚（二甲基硅氧烷）衬底与线光栅（600 nm的脊与600 nm的间距和600 +/- 150 nm的特征高度）诱导hESC对齐和伸长，介导的组织细胞骨架成分，包括肌动蛋白，波形蛋白和α-微管蛋白，并减少增殖。空间极化的γ-微管蛋白复合物也观察到在响应纳米形貌。此外，加入肌动蛋白破坏剂减弱了纳米形貌的排列和增殖效应。这些发现进一步证明了细胞骨架和基质相互作用之间的相互作用作为hESC在纳米形貌上的形态和增殖细胞反应的关键调节剂的重要性。(c)2007爱思唯尔有限公司版权所有。
Mammalian cells respond to their substrates by complex changes in gene expression profiles, morphology, proliferation and migration. We report that substrate nanotopography alters morpohology and proliferation of human embryonic stem cells (hESCs). Fibronectin-coated poly(di-methyl siloxane) substrates with line-grating (600 nm ridges with 600 nm spacing and 600 +/- 150 nm feature height) induced hESC alignment and elongation, mediated the organization of cytoskeletal components including actin, vimentin, and alpha-tubulin, and reduced proliferation. Spatial polarization of gamma-tubulin complexes was also observed in response to nanotopography. Furthermore, the addition of actin disrupting agents attenuated the alignment and proliferative effects of nanotopography. These findings further demonstrate the importance of interplay between cytoskeleton and substrate interactions as a key modulator of morphological and proliferative cellular response in hESCs on nanotopography. (c) 2007 Elsevier Ltd. All rights reserved.