LAT1 is a central transporter of essential amino acids in human umbilical vein endothelial cells.
LAT1 is a central transporter of essential amino acids in human umbilical vein endothelial cells.
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DOI：
10.1254/jphs.13255sc
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发表时间：
2014-04
影响因子：
3.5
通讯作者：
Keitaro Hayashi;P. Jutabha;T. Kamai;H. Endou;N. Anzai
中科院分区：
文献类型：
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作者：
Keitaro Hayashi;P. Jutabha;T. Kamai;H. Endou;N. Anzai
支持血管生成的内皮细胞增殖需要足够的营养供应，因为促进细胞内代谢。然而，很少有人知道的机制，促进营养纳入增殖内皮细胞。在这里，我们表明，L型氨基酸转运蛋白1（LAT1）是一个主要的转运人脐静脉内皮细胞（HUVECs）的必需氨基酸。生长的HUVEC表达一定水平的LAT1。LAT1特异性抑制剂抑制亮氨酸摄取、细胞增殖和HUVEC管形成。因此，LAT1的作用是支持氨基酸的有效摄取，这对于HUVEC血管生成的最佳功能至关重要。
Endothelial cell proliferation supporting angiogenesis requires sufficient nutrient supply because of facilitated intracellular metabolism. However, little is known about the mechanism for the promotion of nutrient incorporation in proliferating endothelial cells. Here we show that L-type amino acid transporter 1 (LAT1) is a major transporter of essential amino acids in human umbilical vein endothelial cells (HUVECs). Growing HUVECs express a certain level of LAT1. A LAT1-specific inhibitor suppressed leucine uptake, cell proliferation, and tube formation of HUVECs. Therefore, LAT1 acts to support effective uptake of amino acids, which is critical for the optimal function of HUVECs for angiogenesis.