Extended Law of Corresponding States Applied to Solvent Isotope Effect on a Globular Protein
Extended Law of Corresponding States Applied to Solvent Isotope Effect on a Globular Protein
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DOI：
10.1021/acs.jpclett.6b00593
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发表时间：
2016-05-05
影响因子：
5.7
通讯作者：
Stradner, Anna
中科院分区：
文献类型：
--
作者：
Bucciarelli, Saskia;Mahmoudi, Najet;Stradner, Anna
用NMR或中子散射等技术研究蛋白质时，经常需要用D2 O部分或完全取代H2O作为溶剂，通常默认这种溶剂取代不会显著改变蛋白质的性质。在这里，我们报告了一个系统的调查溶剂同位素效应的相图的透镜蛋白γ B-晶体蛋白在水溶液中作为一个模型系统表现出液-液相分离。我们表明，所观察到的强烈变化的临界温度T-C可以描述的扩展法律的对应状态的所有H2O/D2 O的比例，其中缩放的温度T-C或减少第二维里系数准确地再现的双结点，旋节，和渗透压缩。这些发现突出了H2O/D2 O取代对γ B-晶状体蛋白性质的影响，并保证进一步调查这种现象的普遍性及其潜在机制。
Investigating proteins with techniques such as NMR or neutron scattering frequently requires the partial or complete substitution of D2O for H2O as a solvent, often tacitly assuming that such a solvent substitution does not significantly alter the properties of the protein. Here, we report a systematic investigation of the solvent isotope effect on the phase diagram of the lens protein gamma B-crystallin in aqueous solution as a model system exhibiting liquid-liquid phase separation. We demonstrate that the observed strong variation of the critical temperature T-c can be described by the extended law of corresponding states for all H2O/D2O ratios, where scaling of the temperature by T-c or the reduced second virial coefficient accurately reproduces the binodal, spinodal, and osmotic compressibility. These findings highlight the impact of H2O/D2O substitution on gamma B-crystallin properties and warrant further investigations into the universality of this phenomenon and its underlying mechanisms.