Radiation Produces Irreversible Chronic Dysfunction in the Submandibular Glands of the Rat
Radiation Produces Irreversible Chronic Dysfunction in the Submandibular Glands of the Rat
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DOI：
10.2174/1874210601206010008
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发表时间：
2012-01-01
影响因子：
1.3
通讯作者：
Elverdin, J. C.
中科院分区：
文献类型：
--
作者：
De la Cal, C.;Fernandez-Solari, J.;Elverdin, J. C.
放射治疗期间暴露于高剂量电离辐射导致唾液腺的严重形态和功能改变，如口干症。在本研究中，我们研究了仅限于头部和颈部的15戈瑞（戈伊）单次辐射剂量对大鼠唾液腺功能（唾液分泌和腺体质量）和组织学的慢性效应。结果表明，去甲肾上腺素（NE）诱导的唾液分泌显着减少，在30，90，180和365天后，单剂量的15戈伊的电离辐射与非辐照动物。最大分泌反应在照射后30和90天减少了33%。有趣的是，在180天观察到唾液对NE的反应的新的下降，并且在照射后365天维持，显示最大反应降低了75%。在照射后180天观察到的唾液分泌的功能下降不仅与腺体质量的减少有关，而且与上皮结构的改变有关，上皮结构表现为导管和腺泡的比例改变、嗜酸性分泌颗粒物质的损失以及腺泡化和纤维化。根据所呈现的结果，我们得出结论，电离辐射产生不可逆的和渐进的改变下颌下腺（SMG）的功能和形态，导致严重的唾液功能减退。
The exposure to high doses of ionizing radiation during radiotherapy results in severe morphological and functional alterations of the salivary glands, such as xerostomia. In the present study we investigated the chronic effect of a single radiation dose of 15 Gray (Gy) limited to head and neck on rat salivary gland function (salivary secretion and gland mass) and histology. Results indicate that norepinephrine (NE)-induced salivary secretion was reduced significantly at 30, 90, 180 and 365 days after the administration of a single dose of 15 Gy of ionizing radiation compared to non-irradiated animals. The maximal secretory response was reduced by 33% at 30 and 90 days post irradiation. Interestingly, a new fall in the salivary response to NE was observed at 180 days and was maintained at 365 days post irradiation, showing a 75% reduction in the maximal response. The functional fall of the salivary secretion observed at 180 days post irradiation was not only associated with a reduction of gland mass but also to an alteration of the epithelial architecture exhibiting a changed proportion of ducts and acini, loss of eosinophilic secretor granular material, and glandular vacuolization and fibrosis. On the basis of the presented results, we conclude that ionizing radiation produces irreversible and progressive alterations of submandibular gland (SMG) function and morphology that leads to a severe salivary hypo-function.