ACE-inhibitors but not endothelin receptor blockers prevent podocyte loss in early diabetic nephropathy
ACE-inhibitors but not endothelin receptor blockers prevent podocyte loss in early diabetic nephropathy
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DOI：
10.1007/s00125-003-1106-8
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发表时间：
2003-06-01
期刊：
影响因子：
8.2
通讯作者：
Amann, K
中科院分区：
文献类型：
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作者：
Gross, ML;El-Shakmak, A;Amann, K
目标/假设。我们研究的目的是探讨选择性 ET-A 受体拮抗剂 LU 135252 单独使用以及与 ACE 抑制剂群多普利联合使用对链脲佐菌素糖尿病大鼠足细胞数量和形态的影响。方法。雄性 Sprague-Dawley 大鼠静脉注射 65 mg 链脲佐菌素。并随后患上糖尿病。动物不进行治疗或每天接受群多普利（0.3mg/kg体重）、LU 135252（50mg/kg体重）或两者的组合。 6个月后实验终止。通过体视学技术评估肾小球几何形状和细胞构成。通过免疫组织化学研究TGF-β、ET-1、PDGF-AB、纤连蛋白、结蛋白和α-平滑肌细胞肌动蛋白的蛋白表达。结果。每个肾小球的足细胞平均数量较低（86+/-17 vs. 138+/-25；p
Aims/hypothesis. It was the aim of our study to investigate the influence of a selective ET-A receptor antagonist LU 135252 alone and in combination with the ACE-inhibitor, trandolapril on podocyte number and morphology in streptozotocin diabetic rats.Methods. Male Sprague-Dawley rats were injected with 65 mg streptozotocin i.v. and subsequently developed diabetes. Animals were left untreated or received daily either trandolapril (0.3 mg/kg body weight), LU 135252 (50 mg/kg body weight) or a combination of both. After 6 months the experiment was terminated. Glomerular geometry and cellularity were assessed by stereological techniques. Protein expression of TGF-beta, ET-1, PDGF-AB, fibronectin, desmin and alpha-smooth muscle cell actin was investigated by immunohistochemistry.Results. The mean number of podocytes per glomerulus was lower (86+/-17 vs. 138+/-25; p