The development of the gut microbiota in rainbow trout (Oncorhynchus mykiss) is affected by first feeding and diet type
The development of the gut microbiota in rainbow trout (Oncorhynchus mykiss) is affected by first feeding and diet type
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DOI：
10.1016/j.actuaculture.2013.12.032
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发表时间：
2014-03-20
期刊：
影响因子：
4.5
通讯作者：
Madsen, L.
中科院分区：
文献类型：
--
作者：
Ingerslev, H. -C.;Jorgensen, L. von Gersdorff;Madsen, L.
使用Illumina HiSeq测序证明了与虹鳟鱼（Oncorhyrichus myki.ss）鱼苗的第一次喂食有关的肠道微生物群的影响。从卵黄囊喂养结束后7周的第一次喂养后，无论是海洋或植物为基础的饮食与或不与益生菌乳酸片球菌管理进行了检查。在第一次进料之前，序列读数的主要部分被分组为可能源自周围水的沉降杆菌属。第一次喂食后，微生物丰度和多样性增加，然后微生物群向厚壁菌门（Firmicutes）转变，以植物为基础的鱼类占优势，向变形菌门（Proteobacteria）转变，以海洋鱼类占优势。首次投喂后，以植物性饲料喂养的鱼类中链球菌、明串珠菌和魏氏球菌的丰度显著较高。根据饮食类型，微生物群分别聚类，但使用PCA分析时，从原生物中只看到较小的影响。大多数检测的免疫基因的组成性转录水平在个体发生转变期间增加，但结果不能解释首次喂养后依赖于饮食处理的微生物群组成的差异。结果表明，虹鳟鱼的肠道在早期状态下被殖民化，但根据饮食类型被引导到新的和不同的方向。(C)2014 Elsevier B.V.保留所有权利，
An influence of the intestinal microbiota in connection to first-feeding of rainbow trout (Oncorhyrichus myki.ss) fry was demonstrated using Illumina HiSeq sequencing. The period from the end of yolk-sac feeding to seven weeks post first-feeding was examined after administration of either a marine- or plant based diet with or without the probiont Pediococcus acidilactici. Before first feeding the main part of the sequence reads grouped to the genus Sediininibacterium probably originating from the surrounding water. The microbial abundance and diversity increased after first-feeding and the microbiota then changed towards phylum Firmicutes dominance for plant based fed fish and towards dominance of phylum Proteobacteria for the marine fed fish. After firstfeeding, there were significantly higher abundances of Streptococcus, Leuconostoc and Weissella in fish fed the plant-based diet. The microbiota clustered separately according to the diet type, but only minor effects were seen from the probiont when using PCA-analysis. The constitutive transcription level of most examined immune genes increased during the ontogenic shift, but the results could not explain the differences in the composition of the microbiota dependent on diet treatment after first-feeding. The results suggest that the intestine of rainbow trout is colonised at an early state, but is guided in new and different directions dependent on the diet type. (C) 2014 Elsevier B.V. All rights reserved,