Cerato-platanin protein is located in the cell walls of ascospores, conidia and hyphae of Ceratocystis fimbriata f. sp platani
Cerato-platanin protein is located in the cell walls of ascospores, conidia and hyphae of Ceratocystis fimbriata f. sp platani
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DOI：
10.1016/j.femsle.2004.03.001
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发表时间：
2004-04-15
影响因子：
2.1
通讯作者：
Scala, A
中科院分区：
文献类型：
--
作者：
Boddi, S;Comparini, C;Scala, A
Cerato-platanin（CP）是一种分子量约为12.4kDa的蛋白质，来源于毛状长囊藻Ceratocystis fumbriata f. platani（Cfp），积累在菌丝体中并位于Cfp子囊孢子、菌丝和分生孢子的细胞壁中，这表明该蛋白质在形成真菌细胞壁中具有作用，除了已知的事实之外，即它在培养早期分泌并促进植物抗毒素合成和/或植物细胞死亡。这一发现是通过三种免疫学技术获得的：定量ELISA，确定菌丝体中CP的量。免疫荧光测定和免疫金标记以确定CP在Cfp细胞中的确切定位。(C)2004年，欧洲微生物学会联合会。Elsevier B. V.出版，保留所有权利。
Cerato-platanin (CP), a protein of about 12.4 kDa from Ceratocystis fumbriata f. sp. platani (Cfp), accumulated in the mycelium and was located in the cell walls of Cfp ascospores, hyphae and conidia suggesting that this protein had a role in forming the fungal cell wall apart from the already known fact that it is secreted early in culture and elicits phytoalexin synthesis and/or plant cell death. The finding was obtained with three immunological techniques: a quantitative ELISA which determines the amount of CP in the mycelium. an immunofluorescence assay, and immunogold labelling to define the exact localization of CP in the Cfp cells. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.