Expression of the p70 chain of the IL‐2 receptor on human lymphoid cells: Analysis using a monoclonal antibody and high‐sensitivity immunofluorescence
Expression of the p70 chain of the IL‐2 receptor on human lymphoid cells: Analysis using a monoclonal antibody and high‐sensitivity immunofluorescence
复制标题
IL-2 受体 p70 链在人淋巴细胞上的表达：使用单克隆抗体和高灵敏度免疫荧光进行分析
作者：
H. Zola;Rebecca J. Purling;L. Koh;M. Tsudo
使用针对白细胞介素2受体（IL-2 R）p70（β）链的单克隆抗体（MoAb）和高灵敏度免疫荧光方法，可以在正常血液样本的大多数淋巴细胞上证明p70，而无需体外刺激。细胞亚群具有高浓度的受体，并且这些细胞具有大颗粒白细胞（LGL）的物理性质。较小的淋巴细胞，虽然较弱，但非常明显地是阳性的，T8细胞染色相对较强，而T4细胞和B细胞被杀死相对较弱。当检查IL-2 R的p55和p70链的表达时，白血病细胞显示出多种表型。在未刺激的淋巴细胞上通过免疫荧光直接显示IL-2 R链具有潜在的临床应用，因为该技术可用于诊断环境。
Using monoclonal antibody (MoAb) against the p70(β)chain of the interleukin‐2 receptor (IL‐2R) and a high‐sensitivity immunofluorescence procedure, p70 can be demonstrated on the majority of lymphocytes from normal blood samples, without in vitro stimulation. A subpopulation of cells bears a high concentration of receptor, and these cells have the physical properties of large granular leucocytes (LGL). The smaller lymphocytes, although weaker, are quite clearly positive T8 cells stain relatively strongly, while T4 cells and B cells slain relatively weakly. Leukaemic cells showed a variety of phenotypes when examined for expression of the p55 and p70 chains of the IL‐2R. The demonstration of IL‐2R chains directly by immunofluorescence on unstimulated lymphocytes has potential clinical applications, since this technique can be used in a diagnostic setting.