A TOBACCO MOSAIC VIRUS-INDUCED TOBACCO PROTEIN IS HOMOLOGOUS TO THE SWEET-TASTING PROTEIN THAUMATIN
A TOBACCO MOSAIC VIRUS-INDUCED TOBACCO PROTEIN IS HOMOLOGOUS TO THE SWEET-TASTING PROTEIN THAUMATIN
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DOI：
10.1038/321531a0
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发表时间：
1986-05-29
期刊：
影响因子：
64.8
通讯作者：
BOL, JF
中科院分区：
文献类型：
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作者：
CORNELISSEN, BJC;VANHUIJSDUIJNEN, RAMH;BOL, JF
烟草花叶病毒（TMV）侵染烟草植株后，导致几种酶的活性增加，并诱导约10种蛋白酶抗性和可溶性atpH3蛋白的新合成。这些蛋白质聚集在细胞间叶空间1,2。发病相关蛋白（PR）的出现与“全身性获得性耐药”现象密切相关，有人认为这类蛋白具有抗病毒功能3,4。之前，我们克隆了三个最小的PR蛋白，PR-1a， -1b和-1c的信使rna的互补dna，这些克隆被用来证明烟草TMV感染后PR-1 mrna的浓度增加了100倍以上5,6。在这里，我们描述了另一个mRNA的cDNA克隆，其合成是由TMV感染诱导的。cDNA测序显示，编码的蛋白质与thumatin高度同源，thumatin是一种来自西非雨林灌木monocotThaumatococcus danielliiBenth果实的强烈甜味蛋白质。烟草中编码一种类似于梭马汀的蛋白质的基因的保存表明，这种编码的蛋白质可能具有比尝甜味更普遍的功能。
Infection of tobacco plants with tobacco mosaic virus (TMV) results in an increase in the activities of several enzymes and induces thede novosynthesis of about 10 proteins that are protease-resistant and soluble atpH3. These proteins accumulate in the intercellular leaf space1,2. The appearance of pathogenesis-related (PR) proteins is closely associated with the phenomenon of ‘systemic acquired resistance’ and it has been suggested that such proteins have an antiviral function3,4. Previously, we cloned complementary DNAs to the messenger RNAs for the three smallest PR proteins, PR-1a, -1b and -1c, and these clones were used to show that there is an increase of more than 100-fold in the concentration of PR-1 mRNAs following TMV infection of tobacco5,6. Here, we describe the cDNA cloning of another mRNA whose synthesis is induced by TMV infection. Sequencing of the cDNA showed that the encoded protein is highly homologous to thaumatin, the intensely sweet-tasting protein from the fruits of the monocotThaumatococcus danielliiBenth, a West African rainforest shrub. The conservation of a gene encoding a thaumatin-like protein in tobacco suggests that the encoded protein may have a more general function than that of being sweet-tasting.