Transplantation of aggregates of synovial mesenchymal stem cells regenerates meniscus more effectively in a rat massive meniscal defect
Transplantation of aggregates of synovial mesenchymal stem cells regenerates meniscus more effectively in a rat massive meniscal defect
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DOI：
10.1016/j.bbrc.2013.05.026
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发表时间：
2013-06-14
影响因子：
3.1
通讯作者：
Sekiya, Ichiro
中科院分区：
文献类型：
--
作者：
Katagiri, Hiroki;Muneta, Takeshi;Sekiya, Ichiro
滑膜来源的间充质干细胞移植是半月板再生的一种可能的治疗方法。我们曾报道关节内注射500万个滑膜间充质干细胞可促进大鼠半月板损伤后的半月板再生。然而，如果需要相似的单位体重的细胞数量，那么在临床情况下制备所需的人MSCs是不现实的。使用骨髓间充质干细胞聚集体可能是解决方案之一。在这里，我们研究了滑膜间充质干细胞聚集物在半月板切除大鼠模型中是否能更有效地再生半月板。将滑膜MSCs总数调整为25000个细胞，并将MSCs或悬浮在PBS中的25000个MSCs聚集体放置在半月板缺损处。悬浮在PBS中的500万MSCs也作为另一种对照。再生半月板在4周时，聚集物组的面积大于悬吊组，组织学表现更接近正常半月板。12周时仍观察骨料移植的效果。在移植相同数量的表达荧光素酶的MSCs时，聚集体组的发光强度在3周及以后仍高于悬浮组。我们证实MSCs以聚集体的形式移植到再生半月板中，局部和部分存在。滑膜间充质干细胞聚集体移植能更有效地修复大鼠半月板损伤。(C)2013 Elsevier Inc.保留所有权利。
Transplantation of mesenchymal stem cells (MSCs) derived from synovium is a possible therapy for meniscus regeneration. We have previously reported that intraarticular injection of 5 million synovial MSCs promoted meniscal regeneration in rat meniscal defects. However, if a similar cell number per body weight were required, preparation of required human MSCs would not be practical in a clinical situation. The use of aggregates of MSCs may be one of the solutions. Here, we investigated whether the use of aggregates of synovial MSCs regenerated meniscus more effectively in a rat meniscectomized model. The total number of synovial MSCs was adjusted to 25,000 cells, and aggregates consisting of MSCs or 25,000 MSCs suspended in PBS were placed on the meniscal defects. Five million MSCs suspended in PBS were also used as another control. For the regenerated menisci, the area was larger and the histological findings were closer to that of the normal meniscus in the aggregate groups than to that in the suspension groups at 4 weeks. The effects of transplantation of aggregates were still observed at 12 weeks. Luminescence intensity remained higher at 3 weeks and thereafter in the aggregate group than in the suspension group when the same number of luciferase expressing MSCs were transplanted. We confirmed that MSCs transplanted as aggregates existed in the regenerated meniscus focally and partially. Transplantation of aggregates of synovial MSCs regenerated meniscus more effectively in a rat massive meniscal defect. (C) 2013 Elsevier Inc. All rights reserved.