ESCHERICHIA-COLI OF HUMAN-ORIGIN BINDS TO CARCINOEMBRYONIC ANTIGEN (CEA) AND NONSPECIFIC CROSSREACTING ANTIGEN (NCA)
ESCHERICHIA-COLI OF HUMAN-ORIGIN BINDS TO CARCINOEMBRYONIC ANTIGEN (CEA) AND NONSPECIFIC CROSSREACTING ANTIGEN (NCA)
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DOI：
10.1016/0014-5793(90)80603-g
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发表时间：
1990-02-26
期刊：
影响因子：
3.5
通讯作者：
WAGENER, C
中科院分区：
文献类型：
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作者：
LEUSCH, HG;HEFTA, SA;WAGENER, C
固定化癌胚抗原（CEA）和非特异性交叉反应抗原（NCA）结合3株e。大肠杆菌的人类起源。这种结合具有剂量依赖性、可饱和性和高亲和力。在10 mM α‐甲基D‐甘露吡喃苷的存在下，细菌与CEA和NCA的结合被完全消除。细菌不与刀豆蛋白a结合。此外，与去糖基化CEA的结合要么不存在，要么显著减少。这些发现表明。大肠杆菌与CEA和NCA中的D‐甘露糖基残基结合。考虑到CEA（结肠上皮刷状缘）和NCA（粒细胞）的组织分布，这些糖蛋白可能参与了细菌的识别。
Immobilized carcinoembryonic antigen (CEA) and non‐specific crossreacting antigen (NCA) bound 3 strains ofE. coliof human origin. The binding was dose dependent, saturable, and of high avidity. Binding of the bacteria to CEA and NCA was completely abolished in the presence of 10 mM α‐methyl D‐mannopyranoside. Bacteria did not bind to concanavalin A. In addition, binding to deglycosylated CEA was either absent or significantly reduced. These findings indicate that theE. colistrains bind to D‐mannosyl residues in CEA and NCA. Considering the tissue distribution of CEA (brush border of colonic epithelium) and NCA (granulocytes), these glycoproteins may be involved in the recognition of bacteria.