Early meiotic-specific protein expression in post-natal rat ovaries.
Early meiotic-specific protein expression in post-natal rat ovaries.
复制标题
DOI：
10.1111/j.1439-0531.2010.01599.x
复制
发表时间：
2010-12
期刊：
影响因子：
--
通讯作者：
P. Zhang;LX Lv;Wanjin Xing
中科院分区：
文献类型：
--
作者：
P. Zhang;LX Lv;Wanjin Xing
最近对小鼠的研究挑战了大多数哺乳动物雌性在出生后卵巢中失去新卵子发生的基本理论。为了提供更多的信息，在其他物种，我们检查出生后的大鼠卵巢组织切片和检测生殖细胞标志蛋白RVLG（大鼠血管样基因），BrdU（5-溴脱氧尿苷）掺入RVLG表达细胞，鉴定生殖细胞进行有丝分裂和减数分裂的卵巢表面上皮（OSE）。用免疫组织化学和Western blotting方法检测视黄酸基因8（STRA 8）和联会复合体蛋白3（SCP 3）刺激下，减数分裂早期特异蛋白减数分裂控制中断1（DMC 1）的表达，用RT-PCR方法检测SCP 1、SCP 3和孢子形成特异蛋白11（SPO 11）的转录。然而，我们未能在OSE中检测到大卵圆形细胞，这可能代表假定的生殖系干细胞（GSC），应该维持新卵子发生，并通过RT-PCR检测到减数分裂特异性基因SCP 1，SCP 3和SPO 11的转录，以及通过Western印迹检测到DMC 1，STRA 8和SCP 3的翻译。我们的数据支持假设，没有发生新的卵子发生在OSE的大鼠出生后的卵巢通过GSC减数分裂。
Recent studies in mice challenged the basic doctrine that most mammalian females lose neo-oogenesis in post-natal ovaries. In order to provide more information in other species, we examined post-natal rat ovaries by histological sections and detected the germline cell marker protein RVLG (rat vasa-like gene), BrdU (5-bromodeoxyuridine) incorporation in RVLG-expressing cells, for identification of germline cells undergoing mitosis and meiosis in the ovarian surface epithelium (OSE). We also detected the expression of early meiotic-specific proteins disruption of meiotic control 1 (DMC1), stimulated by retinoic acid gene 8 (STRA8) and synaptonemal complex protein 3 (SCP3) by immunohistochemical analysis and Western blotting, and the transcript of SCP1, SCP3 and Sporulation-specific protein 11 (SPO11) by RT-PCR in the post-natal ovarian cortex. However we failed in detecting large ovoid cells in the OSE, which may represent the putative germline stem cells (GSCs) that are supposed to sustain neo-oogenesis, and the transcription of the meiotic-specific genes SCP1, SCP3 and SPO11 by RT-PCR as well as the translation of DMC1, STRA8 and SCP3 by Western blotting. Our data support the postulation that there is no neo-oogenesis occurring in the OSE of rat post-natal ovary through meiosis of GSCs.