Normal Location of Thumb/Big Toe may be related to Programmed Cell Death (PCD) in the Preaxial Area of Embryonic Limb
Normal Location of Thumb/Big Toe may be related to Programmed Cell Death (PCD) in the Preaxial Area of Embryonic Limb
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拇指/大脚趾的正常位置可能与胚胎肢体轴前区的程序性细胞死亡（PCD）有关
DOI：
10.1002/ar.21434
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发表时间：
2011
影响因子：
--
通讯作者：
Naruse I
中科院分区：
文献类型：
--
作者：
Kimura S;Yamada S;Naruse I
以新生多指名古屋（Pdn）小鼠的前肢和后肢为研究对象，分析了肢体轴前区程序性细胞死亡（PCD）的作用。特别注意轴前区PCD与第一手指（拇指/大脚趾）的位置和形状之间的关系。尽管Pdn/+小鼠出现了大的、分叉的或重复的拇指/大脚趾，但与+/+小鼠一样，Pdn/+小鼠的手指I（拇指/大脚趾）比其他四个手指更靠近腹侧。另一方面，Pdn/Pdn小鼠的前/后肢的异常轴前指位于远端，并在由指II-V形成的一系列弯曲平面的桡侧/胫骨端对齐;也就是说，在任何轴前指ofPdn/Pdn小鼠中均检测不到正常形状和位置的拇指和大脚趾。在第11 - 12天Pdn小鼠胚胎的肢体发育中，仅在Pdn/+ xPdn/+交配获得的所有胚胎的四分之一中，前/后肢的轴前中胚层未发生PCD。除了指射线II-V，额外的轴前指射线出现在第12天早期这些胚胎的前/后肢的显著轴前扩张中。这些异常的胚胎肢形态与Pdn/Pdn新生小鼠的异常肢形态非常相似。目前的研究结果表明，在轴前区的PCD不仅可以防止额外的数字的形成，但也确定了正常的肢体形态发生的拇指/大脚趾的位置。Anat Rec，2011.© 2011 Wiley‐利斯公司
The forelimbs and hindlimbs of newbornPolydactyly Nagoya(Pdn) mice were examined to analyze the roles of programmed cell death (PCD) in the preaxial region of the limb. Special attention was paid to the relationship between the PCD in the preaxial area and the location and shape of the first digit (thumb/big toe). Although a large, bifurcated or duplicated thumb/big toe appeared inPdn/+ mice, digit I (thumb/big toe) inPdn/+ mice, as in +/+ ones, was located more ventro‐proximally than the other four digits. On the other hand, abnormal preaxial digits of the fore/hindlimb inPdn/Pdnmice lay distally and were aligned at the radial/tibial end of a serial curved plane formed by digits II–V; that is, a thumb and big toe of normal shape and location were not detectable in any preaxial digits ofPdn/Pdnmice. In the limb development ofPdnmouse embryos on Day 11‐12, PCD did not occur in the preaxial mesoderm of fore/hindlimb only in one‐fourths of all embryos obtained byPdn/+ xPdn/+ mating. In addition to digital rays II‐V, extra preaxial digital rays appeared in the prominent preaxial expansion of fore/hindlimbs in these embryos on early Day 12. These abnormal limb configurations in embryos were closely similar to those inPdn/Pdnnewborn mice. The present findings suggest that PCD in the preaxial region not only prevents the formation of extra digits but also determines the location of the thumb/big toe for the normal limb morphogenesis. Anat Rec, 2011. © 2011 Wiley‐Liss, Inc.