Estimation of effects of space radiation using frozen mouse ES cells in ISS
Estimation of effects of space radiation using frozen mouse ES cells in ISS
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DOI：
10.1093/jrr/rrt217
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发表时间：
2014-03
影响因子：
2
通讯作者：
Morita T
中科院分区：
文献类型：
--
作者：
Yoshida K;Hada M;Eguchi-Kasai K;Teramura T;Cucinotta FA;Morita T
评估长期在太空停留期间（包括前往国际空间站 (ISS)、月球或火星的任务）期间太空辐射对人体的影响非常重要。我们使用具有正常核型的小鼠胚胎干（ES）细胞来估计太空辐射的影响。为此，小鼠 ES 细胞已被发射到国际空间站中名为“KIBO”的日本实验舱，并储存在 95°C 的冰箱（MELFI）中，直到返回地球（三年内返回五次）。返回地球后，将通过检测组蛋白H2AX焦点及其染色体畸变来分析太空辐射ES细胞的DNA损伤。 ES细胞也可以显微注射到正常胚胎中并进行体外培养。幸存的胚胎将被植入假怀孕小鼠的子宫中。将检查它们的发育和出生，以估计空间辐射对哺乳动物发育的影响（图1）。
It is important to estimate the influence of space radiation on human body during a longer stay in space, including missions to International Space Station (ISS), the moon, or Mars. We use mouse embryonic stem (ES) cells, which have normal karyotypes to estimate the effects of space radiation. For this purpose, mouse ES cells have been launched to the Japanese experiment module called ‘KIBO’in ISS and stored in a freezer (MELFI) at− 95 C until the return to the Earth (return in five times in 3 years). After returning to the Earth, the DNA damages of space-radiated ES cells will be analyzed by detecting histone H2AX foci and their chromosomal aberrations. The ES cells can also be microinjected into normal embryos and cultured in vitro. The surviving embryos will be implanted into pseudo-pregnant mouse uteruses. Their development and the birth will be examined to estimate the effect of space radiation on mammalian development (Fig. 1).