Polymorphisms in human pre-miRNAs
Polymorphisms in human pre-miRNAs
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DOI：
10.1016/j.bbrc.2005.04.051
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发表时间：
2005-06-17
影响因子：
3.1
通讯作者：
Naraba, H
中科院分区：
文献类型：
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作者：
Iwai, N;Naraba, H
MicroRNAs构成了一类不断增长的非编码rna，它们被认为通过翻译抑制来调节基因表达。microrna最初转录为数百个核苷酸的pri- mirna，然后加工成类似于60个核苷酸的发夹pre- mirna。我们假设pre-miRNA和成熟microRNA的多态性通过影响microRNA的加工和/或靶标选择来改变各种生物过程。在本研究中，我们对96名受试者的173个人类pre-miRNA基因组区域进行了测序，发现10个pre-miRNA发夹区域存在10个多态性。尽管大多数这些多态性似乎对microRNA加工没有影响；我们在成熟的miR-30c-2序列中发现了C到a的多态性。这种多态性可能改变靶标选择，从而产生深远的生物学效应。据我们所知，这是关于pre-miRNAs多态性的第一篇报道。(c) 2005爱思唯尔公司版权所有。
MicroRNAs constitute a growing class of non-coding RNAs that are thought to regulate gene expression via translational repression. microRNAs Eire initially transcribed as several hundred-nucleotide pri-miRNAs and are then processed to similar to 60-nucleotide hairpin pre-miRNAs. We hypothesized that polymorphisms in both pre-miRNA and mature microRNA modify various biological processes by influencing the processing and/or target selection of microRNAs. In the present study, we sequenced 173 human pre-miRNA genome regions in 96 subjects and found 10 polymorphisms in the 10 pre-miRNA hairpin regions. Although most of these polymorphisms seem to have no effect on microRNA processing; we identified a C to A polymorphism in the mature miR-30c-2 sequence. This polymorphism may alter target selection and thus exert profound biological effects. To the best of our knowledge, this is the first report of polymorphisms in pre-miRNAs. (c) 2005 Elsevier Inc. All rights reserved.