In situ generation of the gold nanoparticles bovine serum albumin (AuNPs-BSA) bioconjugated system using pulsed-laser ablation (PLA)
In situ generation of the gold nanoparticles bovine serum albumin (AuNPs-BSA) bioconjugated system using pulsed-laser ablation (PLA)
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DOI：
10.1016/j.matchemphys.2016.04.040
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发表时间：
2016-07-01
影响因子：
4.6
通讯作者：
Mirahmadi-Zare, Seyedeh Zohreh
中科院分区：
文献类型：
--
作者：
Binaymotlagh, Roya;Hadadzadeh, Hassan;Mirahmadi-Zare, Seyedeh Zohreh
本文采用原位法和异位法制备了纳米金-牛血清白蛋白生物复合物。从两种方法的荧光光谱中获得的结合常数（K-b）和吉布斯自由能之间的比较表明，Kb增加，而吉布斯自由能降低，通过减少在原位生成的AuNPs-BSA生物缀合系统中，这表明更小的AuNPs与BSA的相互作用。我们还表明，使用波长为1064 nm的脉冲激光烧蚀对BSA的光降解没有影响。与非原位生物缀合相比，金纳米颗粒与BSA的原位生物缀合产生超小的纳米颗粒。此外，与较大的金纳米颗粒相比，超小纳米颗粒与BSA具有不同的相互作用。由于金纳米簇的重要性，进行理论计算以将Au-42纳米簇对接到BSA中。结果表明，纳米团簇进入BSA并与BSA发生了强烈的相互作用，而不是与BSA表面的相互作用。此外，最好的结合位点内的BSA结构与Au-42的相互作用，确定使用对接计算。(C)2016爱思唯尔B. V.保留所有权利。
In this paper, in situ and ex situ approaches were used for the preparation of gold nanoparticles-bovine serum albumin bioconjugates. The comparison between the binding constants (K-b) and Gibbs free energies obtained from the fluorescence spectra of both approaches reveals that the Kb increases, while the Gibbs free energy decreases by decreasing the size of AuNPs in the in situ generation of the AuNPs-BSA bioconjugated system, which indicates a greater interaction of smaller AuNPs with BSA. We also showed that the use of pulsed-laser ablation with a wavelength of 1064 nm has no effect on the photo degradation of BSA. The in situ bioconjugation of AuNPs with BSA produces ultrasmall nanoparticles in comparison to the ex situ bioconjugation. In addition, the ultrasmall nanoparticles have different interaction with BSA in comparison to the larger AuNPs. Due to the importance of gold nanoclusters, theoretical calculations were performed to dock an Au-42 nanocluster into BSA. The results show that the nanoclusters enter into the BSA and strongly interact with it, rather than the interaction with the BSA surface. Also, the best binding sites inside the BSA structure for the interaction with Au-42 were determined using docking calculations. (C) 2016 Elsevier B.V. All rights reserved.