Preparation and Characterization of Different Sizes of Ethosomes Encapsulated with 5-Fluorouracil and Its Experimental Study of Permeability in Hypertrophic Scar
Preparation and Characterization of Different Sizes of Ethosomes Encapsulated with 5-Fluorouracil and Its Experimental Study of Permeability in Hypertrophic Scar
复制标题
不同粒径5-氟尿嘧啶醇质体的制备、表征及其对增生性疤痕渗透性的实验研究
DOI：
10.1166/jnn.2010.2207
复制
发表时间：
2010-07-01
影响因子：
--
通讯作者：
Cui, Daxiang
中科院分区：
文献类型：
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作者：
Mao, Xiaohui;Wo, Yan;Cui, Daxiang
为了考察不同粒径的氟尿嘧啶醇质体对瘢痕的穿透效果，采用聚碳酸酯膜过滤器挤出法制备了3种不同粒径的醇质体，用透析法考察了它们对氟尿嘧啶（5-FU）的包封率，通过将Rodanmin 600填充到醇质体中并使用共聚焦激光扫描显微镜（CLSM）来分析它们的瘢痕穿透效率。制备的醇质体的直径分别为216 +/- 19 nm、107 +/- 13 nm和65 +/- 10 nm，并表现出良好的分散性。它们对5-FU的包封率分别为12%、34%和41%。结果表明，5-FU的渗透性与醇质体的大小呈线性关系，直径为65 nm的醇质体具有最大的荧光渗透效率，可到达增生性瘢痕真皮深层。结果表明，成功制备了3种不同粒径的5-FU醇质体，粒径为65 nm的5-FU醇质体可高效穿透瘢痕，有望作为抗瘢痕药物载体应用于瘢痕治疗。
With the aim of investigating scar penetration efficiency of different sizes of ethosomes encapsulated with Fluorouracil, three kinds of ethosomes with different sizes were prepared by extruding the vesicles through polycarbonate membrane filters, their encapsulation efficiency of Fluorouracil (5-FU) were investigated by dialysis method, their scar-penetration efficiencies were analyzed by filling Rodanmin 600 into ethosomes and using confocal laser scanning microscopy (CLSM). The prepared ethosomes were 216 +/- 19 nm, 107 +/- 13 nm, and 65 +/- 10 nm in diameter respectively, and exhibited good dispersibility. Their encapsulation efficiency of 5-FU were 12%, 34%, and 41%, respectively. The results indicated that the 5-FU penetration was reversely related to the size of the size of the ethosomes the ethosomes of 65 nm in diameter exhibited maximal fluorescence penetration efficiency which could reach the deep layer of dermis of hypertrophic scar. In conclusion,three different sizes of 5-FU ethosomes were prepared successfully, the ethosomes of 65 nm in diameter with 5-FU can penetrate scar high efficiently, which has potential in application such as anti-scar drug carriers in scar therapy in near future.