Generalized transduction of serotype 1/2 and serotype 4b strains of Listeria monocytogenes
Generalized transduction of serotype 1/2 and serotype 4b strains of Listeria monocytogenes
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DOI：
10.1046/j.1365-2958.2000.01643.x
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发表时间：
2000-01-01
影响因子：
3.6
通讯作者：
Hodgson, DA
中科院分区：
文献类型：
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作者：
Hodgson, DA
这是第一次报告的广义转导革兰氏阳性，食源性病原体单核细胞增生李斯特菌，噬菌体分离自环境和溶源菌，或从其他实验室获得。在测试的59种噬菌体中，34种被证明能够转导。我们利用L.单核细胞增多症在室温下生长，分离的噬菌体在37 ℃下不能生长。因此，在该温度下的转导消除了转导物杀伤和溶原性，包括柠檬酸盐和使用低感染复数也是如此。在每一个特征良好的L.单核细胞增生菌株：EGD，10403，Mack（血型1/2a），L028（血清型1/2c），Scott A（血清型4 b）和来自哈利斯科和哈利法克斯，新斯科舍省爆发的菌株（血清型4 b），P35（phi LMUP 35）是一种特别有用的通用转导噬菌体，具有广泛的宿主范围（所有检测的血清型1/2菌株的75%）。其缺点是它很小，转导相对不频繁。U153（phi CUSI 153/95）比P35大，转导频率增加100倍，但其具有非常窄的宿主范围。我们证明了菌株间的转导，并使用转导来测试各种菌株中转座子插入和突变表型之间的联系。
This is the first report of generalized transduction in the Gram-positive, food-borne pathogen Listeria monocytogenes, Bacteriophages were isolated from the environment and from lysogens, or were obtained from other laboratories. Of the 59 bacteriophages tested, 34 proved to be capable of transduction. We exploited the ability of L. monocytogenes to grow at room temperature and isolated bacteriophages that were incapable of growth at 37 degrees C. Transductions at this temperature therefore eliminated transductant killing and lysogeny, as did inclusion of citrate and the use of a low multiplicity of infection. Transducing bacteriophages were found for each of the well-characterized L. monocytogenes strains: EGD, 10403, Mack (serotype1/2a), L028 (serotype 1/2c), Scott A (serotype 4b) and strains from the Jalisco and Halifax, Nova Scotia outbreaks (serotype 4b), P35 (phi LMUP35) is a particularly useful generalized transducing bacteriophage with a wide host range (75% of all serotype 1/2 strains tested). Its disadvantages are that it is small and transduction is relatively infrequent. U153(phi CUSI153/95) is larger than P35 and transduction frequency increased 100-fold, but it has a very narrow host range. We demonstrated interstrain transduction and used transduction to test linkage between transposon insertions and mutant phenotypes in a variety of strains.