LATERAL MOTION OF FLUORESCENTLY LABELED ACETYLCHOLINE RECEPTORS IN MEMBRANES OF DEVELOPING MUSCLE-FIBERS
LATERAL MOTION OF FLUORESCENTLY LABELED ACETYLCHOLINE RECEPTORS IN MEMBRANES OF DEVELOPING MUSCLE-FIBERS
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DOI：
10.1073/pnas.73.12.4594
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发表时间：
1976-01-01
影响因子：
11.1
通讯作者：
PODLESKI, TR
中科院分区：
文献类型：
--
作者：
AXELROD, D;RAVDIN, P;PODLESKI, TR
本文对原代培养的大鼠肌管表面乙酰胆碱受体（AChR）的横向运动和随年龄变化的分布进行了直接、定量的测量。AChR用四甲基罗丹明标记的α-荧光光漂白恢复技术测定银环蛇毒素和AChR的横向运动。发现了两种共存的不同类型的AChR：移动的、均匀分布的AChR，其在与成肌细胞融合后不久出现在所有肌管上，和非移动的、密集的、高度颗粒状的AChR，其在融合后不久出现在10-60 μ m大小的斑块中，并在肌管变得广泛互连后消失。此外，用四甲基罗丹明-α-标记的AChR的周转的证据表明，在标记后36小时内，在表面荧光的逐渐内化中观察到银环蛇毒素。本文还讨论了肌肉AChR的膜动力学和定位。
Direct, quantitative measurements of the lateral motion and age-dependent distribution of acetylcholine receptors (AChR) on the surface of rat myotubes in primary culture was made. AChR were fluorescently marked with tetramethylrhodamine-labeled .alpha.-bungarotoxin and AChR lateral motion was measured by the fluoresence photobleaching recovery technique. Two coexisting distinct classes of AChR were found: mobile, uniformly distributed AChR that appeared on all myotubes shortly after fusion from myoblasts and immobile, dense, highly granular AChR in patches of 10-60 .mu.m size that appeared shortly after fusion and disappear after myotubes became extensively interconnected. In addition, evidence of turnover of AChR labeled with tetramethylrhodamine-.alpha.-bungarotoxin was seen in the gradual internalization of surface fluorescence within 36 h after labeling. The membrane dynamics and localization of muscle AChR is discussed.