Multicomponent investigations of the hydrogen peroxide- and hydroxyl radical-scavenging antioxidant capacities of biofluids: The roles of endogenous pyruvate and lactate - Relevance to inflammatory joint diseases
Multicomponent investigations of the hydrogen peroxide- and hydroxyl radical-scavenging antioxidant capacities of biofluids: The roles of endogenous pyruvate and lactate - Relevance to inflammatory joint diseases
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DOI：
10.3109/10715769709097781
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发表时间：
1997-01-01
影响因子：
3.3
通讯作者：
Grootveld, M
中科院分区：
文献类型：
--
作者：
Herz, H;Blake, DR;Grootveld, M
生物流体（健康人血清和炎症膝关节滑液）的高场质子 (H-1) 核磁共振 (NMR) 分析已用于评估其中一系列极性、低分子量内源代谢物的过氧化氢 (H2O2) 和羟基自由基（OH 度）清除抗氧化能力。获得的数据表明，丙酮酸消耗H2O2（通过氧化脱羧反应生成乙酸盐和CO2）和乳酸消耗一定程度的OH自由基（生成丙酮酸，随后生成乙酸盐和CO2）可能有助于保护替代生物流体成分（例如大分子）免受活性氧介导的体内氧化损伤。这些结果的机制、生理学和潜在的治疗意义将特别针对炎症性关节疾病进行讨论。
High field proton (H-1) nuclear magnetic resonance (NMR) analysis of biofluids (healthy human blood sera and inflammatory knee-joint synovial fluids) has been employed to evaluate the hydrogen peroxide (H2O2)- and hydroxyl radical (degrees OH)- scavenging antioxidant capacities of a range of polar, low-molecular-mass endogenous metabolites therein. Data obtained indicate that consumption of H2O2 by pyruvate (generating acetate and CO2 via an oxidative decarboxylation reaction) and degrees OH radical by lactate (generating pyruvate, and subsquently acetate and CO2) may serve to protect alternative biofluid components (e.g., macromolecules) against reactive oxygen species-mediated oxidative damage in vivo. The mechanistic, physiological and potential therapeutic implications of these results are discussed with special reference to inflammatory joint diseases.