Polarization of plasma membrane microviscosity during endothelial cell migration.
Polarization of plasma membrane microviscosity during endothelial cell migration.
复制标题
内皮细胞迁移过程中质膜微粘度的极化。
DOI：
10.1016/s1534-5807(03)00397-6
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发表时间：
2004
影响因子：
11.8
通讯作者：
Fox,PaulL
中科院分区：
文献类型：
--
作者：
Vasanji,Amit;Ghosh,PrabarK;Graham,LindaM;Eppell,StevenJ;Fox,PaulL
细胞运动的特征是多个细胞结构的前后极化。我们在这里表明，质膜在移动的内皮细胞（EC）极化，特别是，质膜微粘度（PMM）在细胞的前沿增加。我们的研究表明，胆固醇在这种微粘度梯度的产生中具有重要作用。在体外研究中使用合成的脂质囊泡表明，膜微粘度有一个实质性的和双相的影响肌动蛋白动力学;少量的胆固醇增加肌动蛋白介导的囊泡变形，而大量的完全抑制变形。迁移性EC的实验证实了PMM对肌动蛋白动力学的重要作用。血管生成生长因子刺激的细胞表现出显著增加的细胞膜微粘度，但出乎意料的是，肌动蛋白聚合率下降。我们的研究结果表明，增加PMM在板状伪足可能允许更多的生产性肌动蛋白丝和网状结构的形成，从而提高细胞运动的速率。
Cell movement is characterized by anterior-posterior polarization of multiple cell structures. We show here that the plasma membrane is polarized in moving endothelial cells (EC); in particular, plasma membrane microviscosity (PMM) is increased at the cell leading edge. Our studies indicate that cholesterol has an important role in generation of this microviscosity gradient. In vitro studies using synthetic lipid vesicles show that membrane microviscosity has a substantial and biphasic influence on actin dynamics; a small amount of cholesterol increases actin-mediated vesicle deformation, whereas a large amount completely inhibits deformation. Experiments in migrating ECs confirm the important role of PMM on actin dynamics. Angiogenic growth factor-stimulated cells exhibit substantially increased membrane microviscosity at the cell front but, unexpectedly, show decreased rates of actin polymerization. Our results suggest that increased PMM in lamellipodia may permit more productive actin filament and meshwork formation, resulting in enhanced rates of cell movement.