Generation of a RGS18 gene knockout cell line from a human embryonic stem cell line by CRISPR/Cas9
Generation of a RGS18 gene knockout cell line from a human embryonic stem cell line by CRISPR/Cas9
复制标题
通过 CRISPRCas9 从人胚胎干细胞系中生成 RGS18 基因敲除细胞系
DOI：
10.1016/j.scr.2020.102072
复制
发表时间：
2020-12-01
影响因子：
1.2
通讯作者：
Long, Bing
中科院分区：
文献类型：
--
作者：
Feng, Yashu;Li, Xudong;Long, Bing
RGS18 is a member of the RGS (Regulators of G-protein signaling) protein family, involved in megakaryopoiesis, megakaryocyte differentiation and chemotaxis. Here, we created a RGS18 knockout cell line from a human embryonic stem cell line by CRISPR/Cas9 mediated gene targeting, to further understand roles of RGS18 in these processes. The cell line maintains stem cell morphology and normal karyotype, and retains expression of pluripotent marker genes and differentiation potential in vivo. The RGS18(-/-) cell line will facilitate investigation of the role of RGS18 during multiple cellular processes in human pluripotent stem cell modeled hematopoiesis.
