Novel KDM2B/SAV1 Signaling Pathway Promotes the Progression of Gastric Cancer.
Novel KDM2B/SAV1 Signaling Pathway Promotes the Progression of Gastric Cancer.
复制标题
作者：
萨尔瓦多同源基因1(SAV1)是河马途径的关键成分之一，据报道在不同类型的癌症中发挥肿瘤抑制作用。然而，SAV1在胃癌发生发展过程中的表达及其机制尚不清楚。应用免疫组织化学方法(IHC)检测SAV1和赖氨酸特异性脱甲基酶2B(Kdm2b)在胃癌组织中的表达水平。体外研究SAV1对胃癌细胞增殖、迁移和侵袭的生物学作用。Kdm2b在转录水平上调节SAV1在几个GC细胞系中的表达，并通过分子实验来探讨其可能的机制。SAV1在胃癌组织和细胞系中的表达水平显著降低，与肿瘤侵袭深度、淋巴结转移、TNM分期呈负相关，与胃癌患者的总生存期呈正相关。SAV1过表达抑制了GC细胞的增殖、迁移和侵袭。进一步的机制研究表明，Kdm2b转录调控SAV1的表达，进而调控Hippo信号通路。综上所述，本研究证明Kdm2b转录调控SAV1的表达并促进胃癌的进展。
Salvador homologue 1 (SAV1), which is reported to act as a tumor suppressor in different types of cancer, is one of the key components of the Hippo pathway. However, the expression and mechanisms of SAV1 in the development and progression of gastric cancer (GC) remain to be elucidated. Immunohistochemistry (IHC) was performed in the present study to assess the expression levels of SAV1 and lysine-specific demethylase 2B (KDM2B) in GC tissues. The biological effects of SAV1 on GC cell proliferation, migration, and invasion were studied in vitro. KDM2B transcriptionally regulates SAV1 expression in several GC cell lines, and molecular experiments were performed to investigate underlying mechanisms. The expression level of SAV1 was significantly decreased in GC tissues and cell lines, negatively associated with tumor invasion depth, lymph node metastasis, and TNM stage, and positively associated with the overall survival of patients with GC. SAV1 overexpression inhibited the proliferation, migration, and invasion of GC cells. Further mechanistic studies revealed that KDM2B transcriptionally regulated SAV1 expression and further regulated the Hippo signaling pathway. To conclude, the present study demonstrated that KDM2B transcriptionally regulated SAV1 expression and promoted GC progression.
登录
查看更多内容
影响因子：
--
作者：
Hu G;Dong B;Zhang J;Zhai W;Xie T;Huang B;Huang C;Yao X;Zheng J;Che J;Xu YF
通讯作者：
Xu YF
赖氨酸脱甲基酶 2 (KDM2B) 通过 MOB1 调节 hippo 通路促进胰腺导管腺癌 (PDAC) 进展
DOI：
10.1186/s13046-019-1489-0
发表时间：
2020-01-15
影响因子：
11.3
作者：
Quan, Ming;Chen, Zhiqin;Cui, Jiujie
通讯作者：
Cui, Jiujie
影响因子：
5.2
作者：
Xu M;Xiao J;Chen M;Yuan L;Li J;Shen H;Yao S
通讯作者：
Yao S
影响因子：
--
作者：
Chen M;Wang M;Xu S;Guo X;Jiang J
通讯作者：
Jiang J
影响因子：
4.8
作者：
Oka, Tsutomu;Mazack, Virginia;Sudol, Marius
通讯作者：
Sudol, Marius