Identification of norepinephrine in bovine oviductal fluid by high performance liquid chromatography.
Identification of norepinephrine in bovine oviductal fluid by high performance liquid chromatography.
复制标题
高效液相色谱法鉴定牛输卵管液中的去甲肾上腺素。
DOI：
10.1016/s0024-3205(01)01420-5
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发表时间：
2001
期刊：
影响因子：
6.1
通讯作者：
Killian,GJ
中科院分区：
文献类型：
--
作者：
Way,AL;Barbato,GF;Killian,GJ
精子成熟、受精和早期胚胎发育的最后阶段发生在输卵管的微环境中，对哺乳动物的成功繁殖至关重要。尽管体外研究表明，儿茶酚胺对哺乳动物配子有有益作用，但尚未对天然牛输卵管液中的儿茶酚胺进行鉴定。本研究的目的是确定在牛输卵管液中的儿茶酚胺，并确定是否浓度的变化与发情周期的阶段。通过留置输卵管插管收集输卵管液，并通过高效液相色谱法测定其中是否存在儿茶酚胺。去甲肾上腺素是唯一检测到的儿茶酚胺，浓度范围为0.828 ng/ml - 1117 ng/ml。输卵管液中去甲肾上腺素的存在对应于排卵前、排卵中和排卵后的一段时间，此时血清孕酮水平较低。这是从正常循环奶牛中收集的ODF的一致发现。输卵管液中去甲肾上腺素的潜在功能包括调节输卵管液的形成，诱导精子获能和顶体反应，以及早期胚胎的卵裂。
The final stages of sperm maturation, fertilization and early embryonic development take place in the microenvironment of the oviduct and are essential for successful reproduction in mammals. Although catecholamines have been shown to have beneficial effects on mammalian gametes in vitro, identification of catecholamines in native bovine oviductal fluid has not been studied. The objective of this research was to identify catecholamines in bovine oviductal fluid and to determine whether concentrations of catecholamines change with stage of the estrous cycle. Oviductal fluid was collected via indwelling oviductal cannulae and assayed for the presence of catecholamines by high performance liquid chromatography. Norepinephrine was the only catecholamine detected, in concentrations ranging from 0.828 ng/ml – 1117 ng/ml. The presence of norepinephrine in oviductal fluid corresponded to a period of time just prior to, during, and after ovulation, when serum progesterone levels were low. This was a consistent finding in ODF collected from normally cycling cows. Potential functions of norepinephrine in oviductal fluid include regulation of fluid formation, induction of capacitation and the acrosome reaction in spermatozoa, and cleavage of the early embryo.