Ocular Effects of Glycyrrhizin at Acidic and Neutral pH.
Ocular Effects of Glycyrrhizin at Acidic and Neutral pH.
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DOI：
10.3389/fcimb.2021.782063
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发表时间：
2021
影响因子：
5.7
通讯作者：
Hazlett LD
中科院分区：
文献类型：
--
作者：
Somayajulu M;McClellan SA;Bessert DA;Pitchaikannu A;Hazlett LD
目的：比较酸性和中性pH甘草酸(GLY)对正常小鼠角膜未损伤和损伤后以及感染KEI1025和多重耐药MDR9菌株后的影响。将酸性或中性GLY与磷酸盐缓冲盐水(PBS)局部应用于C57BL/6小鼠正常或损伤的角膜。在未损伤的角膜中，杯状细胞和角膜神经被染色和定量。伤后，用裂隙灯对角膜进行荧光素染色并拍照。用KEI1025和MDR9感染小鼠，对比评价Gly-pH的保护作用。在未损伤的角膜中，应用酸性或中性GLY与PBS相比，球结膜杯状细胞的数量减少，但不改变角膜神经密度。同样应用GLY治疗角膜划痕延迟伤口愈合。在KEI 1025感染后，Gly组和PbS组无一例角膜穿孔；Gly组也减少了板数(中性pH更有效)，MPO和几种细胞因子减少。同样，对于MDR9，GLY在任何一个pH下都具有保护作用，并且也增强了MDR9耐药的莫西沙星的作用。酸性或中性pH GLY使杯状细胞数量减少，但对神经密度无影响。角膜损伤后，pH为1的GLY延迟伤口闭合，(2)感染后，单独使用或与莫西沙星联合使用可减少角膜炎。中性pH值不改变GLY的疗效，临床上应首选GLY。
To test the effects of acidic vs. neutral pH glycyrrhizin (GLY) on the unwounded and wounded normal mouse cornea and after infection with Pseudomonas aeruginosa isolates KEI 1025 and multidrug-resistant MDR9. Acidic or neutral GLY vs. phosphate-buffered saline (PBS) was topically applied to normal or wounded corneas of C57BL/6 mice. In unwounded corneas, goblet cells and corneal nerves were stained and quantitated. After wounding, corneas were fluorescein stained and photographed using a slit lamp. Mice also were infected with KEI 1025 or MDR9 and the protective effects of GLY pH evaluated comparatively. In the unwounded cornea, application of acidic or neutral GLY vs. PBS reduced the number of bulbar conjunctival goblet cells but did not alter corneal nerve density. Similar application of GLY to scarified corneas delayed wound closure. After KEI 1025 infection, none of the GLY vs. PBS-treated corneas perforated; GLY treatment also decreased plate count (neutral pH more effective) and reduced MPO and several cytokines. Similarly, for MDR9, GLY at either pH was protective and also enhanced the effects of moxifloxacin to which MDR9 is resistant. Acidic or neutral pH GLY decreased goblet cell number but had no effect on nerve density. After corneal wounding, GLY at either pH (1) delayed wound closure and, (2) after infection, decreased keratitis when used alone or in combination with moxifloxacin. Neutral pH did not alter the therapeutic effect of GLY and would be preferred if used clinically.