Supercritical fluid extraction of antioxidant compounds from oregano -: Chemical and functional characterization via LC-MS and in vitro assays
Supercritical fluid extraction of antioxidant compounds from oregano -: Chemical and functional characterization via LC-MS and in vitro assays
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DOI：
10.1016/j.supflu.2005.01.003
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发表时间：
2006-08-01
影响因子：
3.9
通讯作者：
Ibanez, Elena
中科院分区：
文献类型：
--
作者：
Cavero, Soffa;Garcia-Risco, Monica R.;Ibanez, Elena
研究了牛至叶作为天然抗氧化剂新来源的可能性。在本研究中，利用中试SFE装置在多种提取条件下（在不同的提取压力和温度下，并考虑添加乙醇作为改性剂）提取牛至叶，目的不仅是了解最佳提取条件，而且是在提取和分离高抗氧化活性产品方面控制过程的变量。采用液相色谱-二极管阵列检测（LC-DAD）和液相色谱-质谱分析（LC-MS）对各实验条件下得到的不同组分进行化学成分表征。九种化合物已初步确定属于一个特定的化合物家族（黄酮、黄烷酮和黄酮醇）；用紫外光谱分析了它们的糖基结构，并根据它们的质谱和分子离子（MH+）主片段给出了初步的取代模式。采用两种不同的体外测定法（1,1-二苯基-2-苦味酰肼（DPPH）抗自由基试验和漂白β -胡萝卜素法）测定其抗氧化活性。(C) 2006 Elsevier B.V.版权所有
The possibility of using oregano leaves as a new source of natural antioxidants has been investigated. In the present study, oregano leaves were extracted using a pilot-scale SFE plant under a wide range of extracting conditions (at different extraction pressures and temperatures and considering the addition of ethanol as modifier) with the objective of knowing not only the best extraction conditions but also the variables that control the process in terms of extraction and fractionation of products of high antioxidant activity. The different fractions obtained at each experimental condition tested were characterized in terms of chemical composition by using liquid chromatography-diode array detection (LC-DAD) and liquid chromatography-mass spectrometry (LC-MS). Nine compounds have been tentatively identified as belonging to a specific family of compounds (flavone, flavanone and flavonol); their aglycon structures have been proposed using UV spectra profile while a tentative pattern of substitution, based on their mass spectra and molecular ion (MH+) major fragments, has been also given. Antioxidant activity was measured using two different in vitro assays (1,1-diphenyl-2-picrylhydrazyl (DPPH) antiradical test and bleaching beta-carotene method). (C) 2006 Elsevier B.V. All rights reserved.