Ploidy in cyanobacteria
Ploidy in cyanobacteria
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DOI：
10.1111/j.1574-6968.2011.02368.x
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发表时间：
2011-10-01
影响因子：
2.1
通讯作者：
Soppa, Joerg
中科院分区：
文献类型：
--
作者：
Griese, Marco;Lange, Christian;Soppa, Joerg
优化了一种新开发的实时荧光定量聚合酶链式反应检测基因组拷贝数的方法，并将其应用于蓝藻。选择了三个种代表一个淡水物种、一个咸水物种和两个广泛使用的实验室物种。聚球藻PCC7942和聚球藻WH7803被发现每个细胞含有3-4个基因组拷贝，因此是寡倍体，证实了早期的报道。聚球藻PCC6803为高度多倍体。游动野生型菌株在指数生长期有218个基因组拷贝，在线性生长期和稳定期有58个基因组拷贝。GT野生型菌株在指数生长期有142个基因组拷贝，在直线生长期和稳定期有42个基因组拷贝。这是所有蓝藻物种中发现的最高数量。值得注意的是，这些数值远远高于20多年前发表的‘Kazusa’毒株的12个基因组拷贝的数值。结果表明，聚球藻PCC6803菌株之间存在差异，其倍性水平受生长阶段的高度调控。所有被调查的蓝藻物种的倍性水平的汇编概述了基因组拷贝数的分布，并表明存在单倍体、寡倍体和多倍体的蓝藻。
A recently developed real-time PCR method for the determination of genome copy numbers was optimized for the application to cyanobacteria. Three species were chosen to represent a fresh water species, a salt water species, and two strains of a widely used laboratory species. Synechococcus PCC 7942 and Synechococcus WH7803 were found to contain 3-4 genome copies per cell and are thus oligoploid, confirming earlier publications. In contrast, Synechocystis PCC 6803 is highly polyploid. The motile wild-type strain contains 218 genome copies in exponential phase and 58 genome copies in linear and in stationary growth phase. The GT wild-type strain contains 142 genome copies in exponential phase and 42 genome copies in linear and stationary growth phase. These are the highest numbers found for any cyanobacterial species. Notably these values are much higher than the value of 12 genome copies published for the 'Kazusa' strain more than 20 years ago. The results reveal that for Synechocystis PCC 6803 strain differences exist and that the ploidy level is highly growth phase-regulated. A compilation of the ploidy levels of all investigated cyanobacterial species gives an overview of the genome copy number distribution and shows that monoploid, oligoploid, and polyploid cyanobacteria exist.