DNA-controlled dynamic colloidal nanoparticle systems for mediating cellular interaction
DNA-controlled dynamic colloidal nanoparticle systems for mediating cellular interaction
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DOI：
10.1126/science.aad4925
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发表时间：
2016-02-19
期刊：
影响因子：
56.9
通讯作者：
Chan, Warren C. W.
中科院分区：
文献类型：
--
作者：
Ohta, Seiichi;Glancy, Dylan;Chan, Warren C. W.
生物系统的精确控制需要开发能够动态改变物理化学性质的材料。受到蛋白质改变其构象以介导功能的能力的启发，我们探索了使用 DNA 作为组装和转化胶体纳米颗粒系统的分子钥匙。该系统由一个被小卫星包围的核心纳米颗粒组成，其构象可以通过脚趾位移机制响应DNA而发生转变。构象变化可以改变组装的纳米系统的光学特性和生物相互作用。荧光团修饰颗粒距离的变化会改变光致发光信号，而通过改变靶向配体的表面显示，细胞靶向效率可提高 2.5 倍。这些概念为设计动态纳米技术系统以导航复杂的生物环境提供了策略。
Precise control of biosystems requires development of materials that can dynamically change physicochemical properties. Inspired by the ability of proteins to alter their conformation to mediate function, we explored the use of DNA as molecular keys to assemble and transform colloidal nanoparticle systems. The systems consist of a core nanoparticle surrounded by small satellites, the conformation of which can be transformed in response toDNAvia a toe-hold displacementmechanism. The conformational changes can alter the optical properties and biological interactions of the assembled nanosystem. Photoluminescent signal is altered by changes in fluorophore-modified particle distance, whereas cellular targeting efficiency is increased 2.5 times by changing the surface display of targeting ligands. These concepts provide strategies for engineering dynamic nanotechnology systems for navigating complex biological environments.