Induction of spermidine/spermine N1-acetyltransferase in rat tissues by polyamines.
Induction of spermidine/spermine N1-acetyltransferase in rat tissues by polyamines.
复制标题
多胺诱导大鼠组织中的亚精胺/精胺 N1-乙酰转移酶。
DOI：
10.1042/bj2310285
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Erwin,BG
中科院分区：
文献类型：
--
作者：
Pegg,AE;Erwin,BG
用亚精胺、精胺或对称去甲亚精胺处理大鼠导致肝、肾和肺中亚精胺/精胺N1-乙酰转移酶活性的显著诱导。这种酶的增加，这是确定独立于其他乙酰化酶，通过使用特定的抗血清，占所有的乙酰化酶活性增加的提取物从这些多胺处理的大鼠。精胺是最活跃的诱导剂，在肝脏中的作用最大。肝脏亚精胺/精胺N1-乙酰转移酶活性增加约300倍，在6小时内的治疗与0.3 mmol/kg剂量的精胺;在肾脏中的活性增加30倍，在这些条件下，在肺中的活性增加15倍。亚精胺/精胺N1-乙酰转移酶活性的增加导致肝腐胺含量的大幅增加和亚精胺的下降。这些变化是由于多胺氧化酶氧化乙酰转移酶形成的N1-乙酰亚精胺。我们的研究结果表明，亚精胺是乙酰化酶/氧化酶途径中高级多胺转化为腐胺的首选底物。多胺诱导的亚精胺/精胺N1-乙酰转移酶可能提供了一种机制，通过这种机制可以去除多余的多胺。
Treatment of rats with spermidine, spermine or sym-norspermidine led to a substantial induction of spermidine/spermine N1-acetyltransferase activity in liver, kidney and lung. The increase in this enzyme, which was determined independently of other acetylases by using a specific antiserum, accounted for all of the increased acetylase activity in extracts from rats treated with these polyamines. Spermine was the most active inducer, and the greatest effect was seen in liver. Liver spermidine/spermine N1-acetyltransferase activity was increased about 300-fold within 6 h of treatment with 0.3 mmol/kg doses of spermine; activity in kidney increased 30-fold and activity in the lung 15-fold under these conditions. The increased spermidine/spermine N1-acetyltransferase activity led to a large increase in the liver putrescine content and a decline in spermidine. These changes are due to the oxidation by polyamine oxidase of the N1-acetylspermidine formed by the acetyltransferase. Our results indicated that spermidine was the preferred substratein vivoof the acetylase/oxidase pathway for the conversion of the higher polyamines into putrescine. The induction of the spermidine/spermine N1-acetyltransferase by polyamines may provide a mechanism by which excess polyamines can be removed.