STM/BP-Like KNOXI Is Uncoupled from ARP in the Regulation of Compound Leaf Development in Medicago truncatula
STM/BP-Like KNOXI Is Uncoupled from ARP in the Regulation of Compound Leaf Development in Medicago truncatula
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STM/BP 类 KNOXI 在调控蒺藜苜蓿复合叶发育中与 ARP 解偶联
DOI：
10.1105/tpc.114.123885
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发表时间：
2014
期刊：
影响因子：
11.6
通讯作者：
Wang Zeng-Yu
中科院分区：
文献类型：
--
作者：
Zhou Chuanen;Han Lu;Li Guifen;Chai Maofeng;Fu Chunxiang;Cheng Xiaofei;Wen Jiangqi;Tang Yuhong;Wang Zeng-Yu
类KNOXI基因对茎尖分生组织的维持至关重要。knoxia的表达域受不对称叶片（asymmetric LEAVES1/ROUGHSHEATH2/PHANTASTICA， ARP）基因调控，与叶片形态相关。在豆科植物的反向重复缺失枝（IRLC）中，leafy同源物（LFY）代替knoxii调控复叶发育。在这里，我们在模型IRLC豆科植物medicago truncatula中表征了arp （PHAN）和shootmeristemless (STM)-andBREVIPEDICELLUS(BP)-like knoxi的功能丧失突变体。arpgenes的功能具有物种特异性。叶片中stm / bp样KNOXIgenes的抑制不受phan介导，也未观察到phanbystm / bp样KNOXIgenes的抑制，表明stm / bp样KNOXIgenes与phaninm解耦。truncatula。此外，对knoxi和theM异位表达的表型输出进行了比较分析。单叶let1 (SINGLE LEAFLET1, SGL1)，揭示了knoxian和SGL1调控叶片发育的平行通路。我们认为sgl1可能以特定阶段的方式调控m叶片发育的不确定状态。truncatula。
Class IKNOTTED-like homeobox (KNOXI) genes are critical for the maintenance of the shoot apical meristem. The expression domain ofKNOXIis regulated byASYMMETRIC LEAVES1/ROUGHSHEATH2/PHANTASTICA(ARP) genes, which are associated with leaf morphology. In the inverted repeat-lacking clade (IRLC) of Fabaceae, the orthologs ofLEAFY(LFY) function in place ofKNOXIto regulate compound leaf development. Here, we characterized loss-of-function mutants ofARP(PHAN) andSHOOTMERISTEMLESS(STM)-andBREVIPEDICELLUS(BP)-like KNOXIin the model IRLC legume speciesMedicago truncatula. The function ofARPgenes is species specific. The repression ofSTM/BP-like KNOXIgenes in leaves is not mediated byPHAN, and no suppression ofPHANbySTM/BP-like KNOXIgenes was observed either, indicating thatSTM/BP-like KNOXIgenes are uncoupled fromPHANinM. truncatula. Furthermore, comparative analyses of phenotypic output in response to ectopic expression ofKNOXIand theM. truncatula LFYortholog,SINGLE LEAFLET1(SGL1), reveal thatKNOXIandSGL1regulate parallel pathways in leaf development. We propose thatSGL1probably functions in a stage-specific manner in the regulation of the indeterminate state of developing leaves inM. truncatula.