Detection of mutation by allele-specific loop-mediated isothermal amplification (AS-LAMP).
Detection of mutation by allele-specific loop-mediated isothermal amplification (AS-LAMP).
复制标题
通过等位基因特异性环介导等温扩增 (AS-LAMP) 检测突变。
DOI：
10.1007/978-1-62703-535-4_10
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发表时间：
2013
影响因子：
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通讯作者：
Kanuka H.
中科院分区：
文献类型：
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作者：
Aonuma H;Badolo A;Okado K;Kanuka H.
为了有效地控制传播病原体的蚊子，准确的蚊子分布监测数据是必不可少的。最近，有报道称，传播疟疾寄生虫的蚊子冈比亚按蚊的kdra突变导致抗药性增加。为了建立一种简便有效的抗药性蚊虫调查方法，应用LAMP技术对冈比亚按蚊的kdr1基因进行了特异性检测。等位基因特异性LAMP(AS-LAMP)方法成功地将杂合子和野生型区分开来。AS-LAMP的稳健性表明，它不仅适用于昆虫的常规鉴定，还适用于其他媒介和农业害虫的鉴定。在这里，我们描述了AS-LAMP检测蚊子突变的方法。
For effective control of pathogen-transmitting mosquitoes, precise surveillance data of mosquito distribution are essential. Recently, an increase of insecticide resistance due to thekdrmutation inAnopheles gambiae, a mosquito that transmits the malaria parasite, has been reported. With the aim of developing a simple and effective method for surveying resistant mosquitoes, LAMP was applied to the allele-specific detection of thekdrgene inAn.gambiae. Allele-specific LAMP (AS-LAMP) method successfully distinguished thekdrhomozygote from the heterozygote and the wild type. The robustness of AS-LAMP suggests its usefulness for routine identification of insects, not only mosquitoes but also other vectors and agricultural pests. Here we describe the method of AS-LAMP to detect mutation inAnophelesmosquitoes.