DEGRADATION OF RUTIN BY ASPERGILLUS FLAVUS: THE CARBON MONOXIDE PRODUCING SYSTEM
DEGRADATION OF RUTIN BY ASPERGILLUS FLAVUS: THE CARBON MONOXIDE PRODUCING SYSTEM
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黄曲霉对芦丁的降解：一氧化碳生产系统
DOI：
10.1139/m63-002
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发表时间：
1963
影响因子：
2.8
通讯作者：
D. Westlake
中科院分区：
文献类型：
--
作者：
F. J. Simpson;N. Narasimhachari;D. Westlake
黄曲霉产生一种适应性酶（或多种酶），其使用1摩尔氧气切割1摩尔槲皮素的杂环以产生1摩尔一氧化碳和1摩尔缩酚酸，2-（3′，4 ′-二羟基苯甲酰氧基）-4，6-二羟基苯甲酸。该酶作用于具有取代基的色酮，如碳2上的甲基或苯基，碳3上的游离羟基，以及碳2和3之间的双键。如果要诱导酶的产生，这些要求也必须存在于底物中。该酶纯化40倍，通过处理与Sephadex，然后通过吸附和洗脱从二乙基氨基乙基纤维素和磷酸钙凝胶，然后通过加热，最后通过沉淀与硫酸铵。这种酶通过加热、其底物或低浓度硫酸铵而稳定。当冻干或储存在−20 °C的溶液中时，它可以长期稳定。活性的最适pH值介于pH 5.0和7.0之间。
Aspergillus flavus produces an adaptive enzyme (or enzymes) that cleaves the heterocyclic ring of 1 mole of quercetin using 1 mole of oxygen to yield 1 mole of carbon monoxide and 1 mole of the depside, 2-(3′,4′-dihydroxybenzoyloxy)-4,6-dihydroxybenzoic acid. The enzyme acts on chromones that possess a substituent such as a methyl or a phenyl group on carbon 2, a free hydroxyl on carbon 3, and a double bond between carbons 2 and 3. These requirements must also be present in a substrate if production of the enzyme is to be induced. The enzyme was purified 40-fold by treatment with Sephadex followed by adsorption on and elution from diethylaminoethyl cellulose and calcium phosphate gel, then by heating, and finally by precipitation with ammonium sulphate. The enzyme is stabilized towards denaturation by heat, by its substrate, or by low concentrations of ammonium sulphate. It is stable for long periods when lyophilized or stored in solutions at −20 °C. The optimum pH for activity lies between pH 5.0 and 7.0...