Obscurin, a giant sarcomeric Rho guanine nucleotide exchange factor protein involved in sarcomere assembly.
Obscurin, a giant sarcomeric Rho guanine nucleotide exchange factor protein involved in sarcomere assembly.
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DOI：
10.1083/jcb.200102110
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发表时间：
2001-07-09
期刊：
影响因子：
--
通讯作者：
Gautel M
中科院分区：
文献类型：
--
作者：
Young P;Ehler E;Gautel M
脊椎动物横纹肌的非凡秩序被认为归功于巨大的模块化信号蛋白--肌动蛋白和星云蛋白的分子标尺功能。这两种蛋白质被认为代表了脊椎动物肌肉中蛋白质进化的独特结果。本文报道了脊椎动物肌肉中编码在染色体1q42上的第三个巨型蛋白Oblcurin的鉴定结果。Obscurin的∼为800kD，在骨骼肌和心肌中特异表达。Oblcurin的完整cDNA序列呈现模块化结构，由具有多种剪接变体的细胞内免疫球蛋白(Ig)或纤维连接蛋白-3样结构域组成。一个大的暗区显示了串联Ig结构域的模块化结构，这让人想起Titin的弹性区域。Oblcurin的COOH末端区域通过两个特定的Ig样结构域与Titin的NH2末端Z盘区相互作用。这两种蛋白质在肌原纤维形成过程中共同组装。在肌原纤维形成的过程中，所有的隐蔽蛋白表位都可以在M带检测到。在COOH末端存在一个钙调蛋白结合的IQ基序和一个Rho鸟嘌呤核苷酸交换因子结构域，这表明Oblcurin参与了肌节中的钙/钙调蛋白以及G蛋白偶联的信号转导。
Vertebrate-striated muscle is assumed to owe its remarkable order to the molecular ruler functions of the giant modular signaling proteins, titin and nebulin. It was believed that these two proteins represented unique results of protein evolution in vertebrate muscle. In this paper we report the identification of a third giant protein from vertebrate muscle, obscurin, encoded on chromosome 1q42. Obscurin is ∼800 kD and is expressed specifically in skeletal and cardiac muscle. The complete cDNA sequence of obscurin reveals a modular architecture, consisting of >67 intracellular immunoglobulin (Ig)- or fibronectin-3–like domains with multiple splice variants. A large region of obscurin shows a modular architecture of tandem Ig domains reminiscent of the elastic region of titin. The COOH-terminal region of obscurin interacts via two specific Ig-like domains with the NH2-terminal Z-disk region of titin. Both proteins coassemble during myofibrillogenesis. During the progression of myofibrillogenesis, all obscurin epitopes become detectable at the M band. The presence of a calmodulin-binding IQ motif, and a Rho guanine nucleotide exchange factor domain in the COOH-terminal region suggest that obscurin is involved in Ca2+/calmodulin, as well as G protein–coupled signal transduction in the sarcomere.
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DOI：
10.1083/jcb.132.5.835
发表时间：
1996-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Benian GM;Tinley TL;Tang X;Borodovsky M
通讯作者：
Borodovsky M
影响因子：
3.5
作者：
Gautel, M;Goulding, D
通讯作者：
Goulding, D
影响因子：
14.9
作者：
DEVEREUX, J;HAEBERLI, P;SMITHIES, O
通讯作者：
SMITHIES, O
DOI：
10.1073/pnas.93.11.5466
发表时间：
1996-05-28
影响因子：
11.1
作者：
Debant, A;SerraPages, C;Streuli, M
通讯作者：
Streuli, M
影响因子：
7.8
作者：
GROVE, BK;KURER, V;EPPENBERGER, HM
通讯作者：
EPPENBERGER, HM