REPLICATION OF BORNA DISEASE VIRUS IN CELL-CULTURES
REPLICATION OF BORNA DISEASE VIRUS IN CELL-CULTURES
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DOI：
10.1007/bf02122849
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发表时间：
1980-01-01
影响因子：
5.4
通讯作者：
ROTT, R
中科院分区：
文献类型：
--
作者：
HERZOG, S;ROTT, R
来自马或兔感染脑组织的博纳病病毒可在兔或大鼠的胚胎脑细胞中生长，病毒产量高。细胞持续感染，可进行继代培养而不丧失感染性。受感染的兔脑（ERB）细胞与GMK、Vero（非洲绿猴肾）或MDCK（犬肾）细胞共培养，导致持续感染的细胞系。共培养后的BD病毒在MDCK细胞中生长，适应了这种细胞类型，可以直接用于进一步感染MDCK细胞。
Borna disease (BD) virus from infected brain tissue of horses or rabbits could be grown in embryonic brain cells from rabbits or rats with high virus yields. The cells became persistently infected and could be subcultivated without loss of infectivity. Cocultivation of infected rabbit brain (ERB) cells with GMK, Vero [African green monkey kidney], or MDCK [Canine kidney] cells led to persistently infected cell lines. BD virus grown in MDCK cells after cocultivation became adapted to this cell type and could be used directly for further infection of MDCK cells.